奶牛乳铁蛋白基因5′侧翼区遗传多态性及其与乳腺炎关联性分析  

Genetic polymorphism in 5′-flanking region of the lactoferrin gene and its associations with mastitis in Chinese Holstein cows

作　　者：王洪梅[1] 孔振兴[1] 王长法[1] 黄金明[1] 李秋玲[1] 侯明海[1] 李建斌[1] 仲跻峰[1] 

机构地区：[1]山东省农业科学院奶牛研究中心,济南250100

出　　处：《遗传》2009年第4期393-399,共7页Hereditas（Beijing)

基　　金：国家高技术研究发展计划项目(863计划)(编号:2006AA10Z1D9);公益性行业科研专项(编号:nyhyzx07-036-09);山东省良种工程(编号:2006LZ10-04);山东省科技攻关项目(编号:2006GG2209011)资助

摘　　要：牛乳铁蛋白(Lactoferrin,LF)是保护乳腺组织的防御因子之一,是具有多种功能的糖蛋白。关于牛LF基因的多态性研究的报道较多,但其多态性与奶牛乳腺炎相关性的研究较少。文章采用PCR-RFLP、CRS-PCR对268头中国荷斯坦牛LF基因启动子区的-926(G/A)、-915(T/G)、-478(/G)、+72(T/C)突变进行基因型分型,应用最小二乘线性模型分析LF基因多态性与体细胞评分(Somaticcellscore,SCS)的相关性。结果表明,新发现+72(T/C)和-478(/G)对SCS有显著影响,而其他两个位点对SCS影响不显著(P>0.05)。+72(T/C)的AB基因型是优良基因型,其个体的SCS值均显著低于AA型(P<0.01)、BB型个体(P<0.05)。-478(/G)位点的C等位基因是优良的等位基因,CC基因型个体的SCS值极显著低于CD、DD基因型个体(P<0.01)。因此,LF基因+72(T/C)的AB基因型和-478(/G)位点的CC基因型均是奶牛乳腺炎抗性的优良基因型,可作为分子标记应用于奶牛乳腺炎抗性筛选。Bovine lactoferrin （LF） is a multifunctional glycoprotein found in milk, which acts mainly as a defense factor in the mammary gland. Polymorphism has been found in the bovine LF gene. However, there is no report on genetic polymorphism of LF gene and its associations with mastiffs in dairy cattle. In this study, the promoter fragment of LF gene con- taining -926（G/A）, -915（T/G）, -478（/G）, and ＋72（T/C） mutations were genotyped by the PCR-RFLP and CRS-PCR method. Two hundred and sixty-eight Chinese Holstein cows were screened. Least square linear model （LSM） analysis was applied to evaluate the associations of LF gene with somatic cell score （SCS）. The results indicated that the SCS was significantly affected by --478（/（3） and ＋72（T/C）, but not by the other two loci （P 〉0.05）. The SCS of cow with genotype AB in ＋72（T/C） position was significantly lower than that of genotype AA （P〈0.01） or AB （P〈0.05）. In position -478（/G）, the cow with genotype CC showed significantly lower SCS in contrast to cow with genotype CD and DD （P 〈 0.01）. In conclusion,genotype AB in position ＋72（T/C） and genotype CC in position -478（/G） of LF gene were advantageous genotype, which can be used as candidate markers for mastiffs resistance selection in dairy cattle.

关 键 词：乳铁蛋白基因5’侧翼区 遗传多态性 乳腺炎 体细胞评分 中国荷斯坦牛 

分 类 号：S858.23[农业科学—临床兽医学]