盐酸小檗碱抗假丝酵母菌及抗肿瘤活性研究  

Study on antifungal activities of hydrochloric berberine against oral Candida and the inhibitory effects on growth of human tonge carcinoma cells Tca8113 in vitro

作　　者：赵民[1] 迟华基[2] 牟文丽[1] 崔彬[1] 

机构地区：[1]山东大学附属省立医院,250021 [2]山东中医药大学

出　　处：《实用口腔医学杂志》2009年第2期193-196,共4页Journal of Practical Stomatology

摘　　要：目的:研究盐酸小檗碱抗假丝酵母菌及抗肿瘤活性,为临床用药提供依据。方法:依据NCCLS的M27-A2标准方案测定标准株对盐酸小檗碱的药物敏感性;MTT法测定盐酸小檗碱对细胞生长的抑制作用;流式细胞仪检测盐酸小檗碱对人舌癌细胞Tca8113凋亡率的影响。结果:盐酸小檗碱具有抗假丝酵母菌活性,能抑制Tca8113细胞生长;浓度为160、80、40、20、10μg/ml的盐酸小檗碱作用于Tca8113细胞48h后凋亡率分别为32.3%、24.6%、13.3%、6.35%、6.51%,对照为8.46%。结论:盐酸小檗碱(C20H18ClNO4)体外具有抗假丝酵母菌活性,且能抑制舌癌细胞Tca8113的生长,诱导舌癌细胞Tca8113的凋亡。Objective ：To study antifungal activities of hydrochloric berherine（ C20H18 ClNO4 ） against oral Candida spp by NCCLS M27-A2 method and to study the growth inhibition and apoptosis induction effects of hydrochloric berberine on human tonge carcinoma cells Tca8113 in vitro. Methods：The in vitro susceptibilities of Candida spp to fluconazole and hydrochloric berherine were determined by NCCLS M27-A2 method. Tca8113 cell proliferation ability was measured by MTT assay. Apoptotic rate of the cells was analyzed by flow cytometry. Results： Hydrochloric berberine exhibited antifungal activity. MTT assay results showed that hydrochloric berberine inhibited Tca8113 cells growth. After treated with 160, 80, 40, 20, 10 μg,/ml hydrochloric berherine for48 h, FCM assay revealed that the apoptotie rate were 32.3%, 24.6% , 13.3%, 6.35%, 6.51% respectively. Conclusion： Hydrochloric berberine might be used for the prevention and treatment of oral eandidiasis. Hydrochloric berberine has inhibitory effects on the growth of Tca8113 and induces the apoptosis of human tonge carcinoma cells Tca8113.

关 键 词：盐酸小檗碱 口腔假丝酵母菌 药物敏感性实验 抗肿瘤作用 

分 类 号：R96[医药卫生—药理学] R730.5[医药卫生—药学]