梅花鹿茸提取物及TGF-β1对山羊切割后胚胎修复和发育的影响  

Influence on the Repair and Development of Deer Antler’s Extraction and TGF-β1 of the Goat Embryos after Cutting

作　　者：张锐[1] 俞颂东[2] 

机构地区：[1]漳州师范学院生物科学与技术系,福建漳州363000 [2]浙江大学饲料科学研究所,浙江杭州310029

出　　处：《漳州师范学院学报（自然科学版）》2009年第1期80-83,共4页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：漳州师范学院科研基金资助项目(SK08018)

摘　　要：为了修复切割后的山羊胚胎,本研究将梅花鹿茸提取物和转化生长因子(TGF-β1)分别以不同的浓度添加到含10%FBS的PBS基础培养液中.对桑椹期胚胎进行切割后,在5%CO2,95%空气,38.5℃,湿度100%的培养箱中进行培养1-3天.结果表明,相对于转化生长因子而言,鹿茸提取物对切割后的胚胎具有更好的修复作用,并能提高切割胚的囊胚期发育率.In order to repair the goat embryos alter cutting, the research will add the deer antler＇s extraction and transforming growth factor （TGF-β1） by different concentrations to the basis of PBS culture medium containing 10% FBS. The morulae embryos after cutting will be cultured in the 5% CO2, 95% air, 38.5℃, humidity 100% incubator for 1-3 days. The results showed that compared with the transforming growth factor, deer antler＇s extraction on the embryo after cutting has a better repair, and improves the development rate of the embryos after cutting on the blastocyst.

关 键 词：鹿茸提取物 TGF—β1 山羊 胚胎 

分 类 号：S814.8[农业科学—畜牧学]