ELISA方法测定血清中Hib多糖抗体含量的条件比较研究  

Comparative study of test conditions of ELISA for measuring the amount of antibody against Haemophilus influenzae type b polysaccharide

作　　者：李红[1] 唐静[1] 李茂光[1] 李亚南[1] 何莉[1] 叶强[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《微生物学免疫学进展》2009年第1期36-38,共3页Progress In Microbiology and Immunology

摘　　要：分别以牛血清白蛋白和人血清白蛋白作为封闭液的主要成分测定7份血清中抗b型流感嗜血杆菌荚膜多糖的抗体含量,结果显示,两组数据之间没有显著性差异(P>0.05)。分别用HRP标记的羊抗人IgG和HRP标记的鼠抗人IgG测定该7份血清,结果显示两组结果无显著性差异(P>0.05)。同时,将血清反应的温度和时间由37℃1.5h改为4℃16h(过夜),结果显示两者无显著性差异(P>0.05)。从而优化了该方法。Bovine serum albumin and human serum albumin were used as blocking reagent respectively to measure the amount of IgG against capsular polysaccharide （ CPS ） of Haemophilus influenzae type b （ Hib ） in seven human serum samples. Two kinds of HRP-labelled antibody were used respectively to assay IgG against CPS of Hib contained in the same seven samples. The incubate tempreture and time were also changed from 37℃ for 1.5h to 4℃ for 16h （ over night）. The results showed that there is no significant statistical difference between the two groups which were compared （ P 〉 0.05 ）. Thus the comparative study can give more choices in the assay of measuring the level of Hib antibody.

关 键 词：B型流感嗜血杆菌 酶联免疫测定 抗体 

分 类 号：R446.6[医药卫生—诊断学]