敌百虫暴露对孕鼠脏器及胎鼠DNA和生长发育的影响  

Effects of trichlorfon exposure on maternal organ,fetal DNA and development in mice

作　　者：丁瑜[1] 周淑芳[1] 沈莉[1] 高宇[1] 施蓉[1] 周义军[1] 田英[1] 

机构地区：[1]上海交通大学公共卫生学院,上海200025

出　　处：《上海交通大学学报（医学版）》2009年第3期248-251,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30571542;30872086);上海市教委基金(07ZZ31);上海市科委研发基地项目(07dz22013);上海市卫生局基金(08GWD11;08GWZX0302;08GWQ002)~~

摘　　要：目的研究低剂量有机磷农药敌百虫暴露对孕鼠脏器及胎鼠DNA和生长发育的影响。方法雌性ICR小鼠,随机分为空白对照组、10mg/kg敌百虫染毒组和50mg/kg敌百虫染毒组。每组至少10只孕鼠,孕前和孕期以饮水方式染毒共30d,于孕第17天断髓处死,比较孕鼠染毒前后体质量和脏器系数;观察胎鼠生长发育情况,并行单细胞凝胶电泳实验检测胸腺DNA损伤(DNA百分含量、尾长、尾矩、Olive尾矩、细胞拖尾率)。结果染毒组孕鼠除肝脏脏器系数较对照组显著降低外(P<0.01),体质量及胸腺和卵巢等脏器系数与对照组比较差异均无统计学意义(P>0.05)。50mg/kg染毒组胎鼠体质量较对照组明显降低(P<0.05);而染毒组的活胎、死胎及畸形发生率等与对照组比较差异均无统计学意义(P>0.05)。染毒组胎鼠胸腺DNA损伤各项指标均较对照组严重(P<0.05或P<0.01)。结论经口敌百虫暴露(10、50mg/kg),在未对孕鼠产生明显毒性的情况下,可引起胎鼠生长发育延缓和DNA遗传学损伤。Objective To investigate the effects of low dose trichlorfon exposure on the maternal organ,fetal DNA and development in mice. Methods Pregnant ICR mice were randomly divided into control group,10mg/kg trichlorfon group and 50mg/kg trichlorfon group,and were administrated 0,10 and 50mg/kg trichlorfon,respectively by water consumption for 30d. All mice were sacrificed on gestation d 17,and body weight and organ coefficient of pregnant mice were compared among groups before and after exposure to trichlorfon. Fetal development was observed,and thymus DNA damage of fetuses （DNA percent,tail length,tail moment,Oliver tail moment and comet cell percent） were measured with single cell gel electrophoresis assay. Results Compared with control group,the organ coefficient of liver was significantly lower in 10 and 50mg/kg trichlorfon groups （P〈0.01）,while there was no significant difference in the other organ coefficient and body weight among them. There was no significant difference in the incidences of live births,still births and malformed stillbirths among control group and 10 and 50mg/kg trichlorfon groups,while the weight of fetuses was significantly lower in 50mg/kg trichlorfon group than that in control group （P〈0.05）. More severe thymus DNA damage were detected in fetuses of 10 and 50mg/kg trichlorfon groups than in those of control group （P〈0.05 or P〈0.01）. Conclusion Maternal oral trichlorfon exposure （10 or 50mg/kg） may cause fetal development delay and DNA damage while there is no obvious maternal toxicity to pregnant mice.

关 键 词：敌百虫 生长发育 DNA 损伤 胚胎 孕鼠 

分 类 号：R114[医药卫生—卫生毒理学]