3种猪苓营养菌丝酯酶和过氧化物酶的同工酶分析  

Peroxidase Isozyme and Esterase Isoenzyme in Three Nutrient Mycelium of Polyporus umbellatus

作　　者：陈文强[1] 邓百万[1] 丁锐[1] 刘开辉[1] 吕旬丽[2] 解修超[1] 丁小维[1] 彭浩[1] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723001 [2]陕西科技大学附中,陕西咸阳712081

出　　处：《食品与生物技术学报》2009年第2期234-239,共6页Journal of Food Science and Biotechnology

基　　金：陕西省教育厅重点实验室重点科研项目(04JS32)

摘　　要：采用聚丙烯酰胺凝胶垂直板电泳（PAGE）对3种猪苓营养菌丝中的酯酶（EST）与过氧化物酶（POD）的同工酶进行了分析。结果表明，3种猪苓营养菌丝的酯酶同工酶和过氧化物酶同工酶活性相近。酯酶同工酶共显一种谱型，5条谱带，Rf值集中在0．22～0.78间；过氧化物酶同工酶共显3种谱型，酶谱条带数在4～9条间，P．umbellatusLBZ，P．umbellatusLBJ，P．umbellatusLBT的过氧化物酶酶谱条带数依次为8条，4条，9条，Rf值集中在0．08～0．69间。聚类分析显示，P．umbellatusLBJ较其他2种猪苓亲缘关系较远，P．umbellatusLBZ和P．umbellatusLBT亲缘关系较近。In this manuscript, Polyacrylamide gel electrophoresis（PAGE） was adopted to study esterase isozyme and peroxidase isozyme in three different Polyporus umbellatus strains. The three strains are presented similar esterase isozyme and peroxidase isozyme activites, including no their own key bands. Among the three tested Polyporus umbellatus samples, five esterase isozyme bands appeared on the gel, forming one zymogram types. The peroxidase isozyme bands for the three strains were four--nine, eight ones for Polyporus umbellatus LBZ, four ones for Polyporus umbellatus LBJ and nine ones for Polyporus umbellatus LBT, with Rf values were between 0. 08 and 0. 69. Cluster analysis indicated Polyporus umbellatus LBJ had a distant genetic relationship with the other two strains, but Polyporus umbellatus LBJ had a closer genetic relationship with Polyporus umbellatus LBZ and Polyporus umbellatus LBT.

关 键 词：猪苓 酯酶 过氧化物酶 同工酶 

分 类 号：R284[医药卫生—中药学]