Indirect Enzyme-Linked Immunosorbent Assay Based on the Nucleocapsid Protein of SARS-like Coronaviruses  

作　　者：Jun-fa YUAN Yan LI Hua-jun ZHANG Peng ZHOU Zhen-hua KE Yun-zhi ZHANG Zheng-li SHI 

机构地区：[1]Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China [2]Yunnan Institute ofEndemic Diseases Control and Prevention, Dali 671000, China

出　　处：《Virologica Sinica》2009年第2期146-151,共6页中国病毒学（英文版）

基　　金：The State Key Program for Basic Research Grant (2005CB523004) ;The Knowledge InnovationProgram Key Project (KSCX1-YW-R-07).

摘　　要：The nucleocapsid protein(N) is a major structural protein of coronaviruses. The N protein of bat SARS-like coronavirus(SL-CoV) has a high similarity with that of SARS-CoV. In this study,the SL-CoV N protein was expressed in Escherichia coli,purified and used as antigen. An Indirect Enzyme-Linked Immunosorbent Assay(indirect ELISA) was developed for detection of SARS-or SL-CoV infections in bat populations. The detection of 573 bat sera with this indirect ELISA demonstrated that SL-CoVs consistently circulate in Rhinilophus species,further supporting the proposal that bats are natural reservoirs of SL-CoVs. This method uses 1-2 μl of serum sample and can be used for preliminary screening of infections by SARS-or SL-CoV with a small amount of serum sample.The nucleocapsid protein （N） is a major structural protein of coronaviruses. The N protein of bat SARS-like coronavirus （SL-CoV） has a high similarity with that of SARS-CoV. In this study, the SL-CoV N protein was expressed in Escherichia coli, purified and used as antigen. An Indirect Enzyme-Linked Immunosorbent Assay （indirect ELISA） was developed for detection of SARS- or SL-CoV infections in bat populations. The detection of 573 bat sera with this indirect ELISA demonstrated that SL-CoVs consistently circulate in Rhinilophus species, further supporting the proposal that bats are natural reservoirs of SL-CoVs. This method uses 1-2 μl of serum sample and can be used for preliminary screening of infections by SARS- or SL-CoV with a small amount of serum sample.

关 键 词：SARS-like CoV Nucleocapsid protein Indirect ELISA 

分 类 号：S858.31[农业科学—临床兽医学] TS941.724[农业科学—兽医学]