肺癌与肺良性疾病血清蛋白质双向电泳差异分析  

Difference in Two-Dimensional Electrophoresis Maps of Serum Proteins between Lung Cancer and Benign Pulmonary Disease

作　　者：李雪华[1] 刘静[2] 何瑞波[3] 容哲[2] 李欣[4] 

机构地区：[1]武警医学院病理学教研室、天津市职业与环境危害生物标志物重点实验室,300162 [2]武警医学院病理学教研室,300162 [3]武警医学院基础医学实验教研室,300162 [4]南方医科大学肿瘤研究所

出　　处：《天津医药》2009年第3期165-166,共2页Tianjin Medical Journal

基　　金：国家重点基础研究发展计划973项目(项目编号:2001CB510208);天津市科委自然科学基金面上项目(项目编号:06YFJMJC09000);武警医学院科研基金(项目编号:WY2006-8)

摘　　要：目的:分析肺癌与肺良性疾病差异表达的血清蛋白质。方法:以肺癌和肺良性疾病患者血清标本为研究对象,利用双向电泳(2-DE)分离2组血清蛋白质,银染显色,Umax扫描仪获取图像,Melanie4软件分析图像,寻找肺癌相关差异蛋白质。结果:建立了较稳定的肺癌和肺良性疾病血清蛋白质2-DE图谱,平均蛋白质点数约400个。软件分析显示2组共有6个差异蛋白质点,肺癌组量上调的差异点5个,量下调的差异点1个。结论:差异蛋白质的分离为进一步的肺癌相关血清蛋白质的鉴定和其他肿瘤血清蛋白质组学研究奠定了良好的工作基础。Objective：To analyze the differentially expressed serum proteins between lung cancer and benign pulmonary disease. Methods ：The sera samples from patients with tung cancer or benign pulmonary disease patients were studied simultaneously, separating the proteins by two-dimensional gel electrophoresis （2-DE） .The gels were stained by sliver, scanned by Umax scanner, analyzed by Melanie 4 software to find differential protein spots associated with lung cancer.Results：Two-dimensional electrophoresis （2-DE） maps were constantly obtained from sera of lung cancer and benign pulmonary disease groups. The average protein spots were 400. Maps analysis by software revealed 6 differential protein spots between two groups. Five differential protein spots were highly expressed in lung cancer group and 1 protein spot were highly expressed in benign pulmonary disease group. Conclusion： The separation of differentially expressed proteins from lung cancer serum will be useful for further identifying proteins associated with lung cancer and other related tumor serum proteomic researches.

关 键 词：肺肿瘤 肺疾病 电泳 凝胶 双向 血蛋白质类 

分 类 号：R446.112[医药卫生—诊断学] R734.2[医药卫生—临床医学]