高浓度Hcy致血管内皮损伤的机制  

Mechanisms of High Concentration of Hcyomocysteine in Endothelium Damage

作　　者：宋衍秋[1,2] 赵莉莉[2] 李汇[3] 毛用敏[2] 崔让庄[2] 

机构地区：[1]天津医科大学,300070 [2]天津市胸科医院 [3]天津医科大学总医院

出　　处：《天津医药》2009年第3期204-206,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目(项目编号:033609911)

摘　　要：目的:探讨高浓度同型半胱氨酸(homocysteine,Hcy)对人脐静脉内皮细胞(HUVEC)CRL-1730一氧化氮合酶(eNOS)mRNA表达的影响。方法:用不同浓度Hcy(0～5.0mmol/L)刺激HUVEC CRL-1730,分别作用1～24h,收集细胞,提取总RNA,逆转录得到cDNA,PCR扩增eNOS基因片段,2%琼脂糖凝胶电泳分离PCR产物,计算相对含量。结果:随着Hcy浓度增高HUVEC CRL-1730eNOS mRNA表达逐渐降低;随着Hcy作用时间的延长,HUVEC CRL-1730eNOS mRNA表达降低。病理浓度(0.1mmol/L)及毒性药理浓度(5.0mmol/L)Hcy与生理浓度Hcy(0.01mmol/L)相比可显著降低HUVEC CRL-1730eNOS mRNA的表达。结论:高浓度Hcy可能通过降低eNOS水平,减少NO合成,导致血管内皮功能损伤。Objective： To investigate the effect of homocysteine （Hcy） at different concentrations on the eNOS mRNA in cultured human umbilical vein endothelial cells （HUVEC）, CRL-1730. Methods：The HUVEC CRL-1730 was cultured with different concentrations of Hcy （0 to 5.0 mmol/L）for 1 to 24 hours, and then the cells were collected to isolate the total RNA. RT-PCR and 2% agarose gel electrophoresis were performed to get the eNOS fragments. The results were calculated. Results： The eNOS mRNA was significantly depressed with gradually increased concentration of Hcy. Hcy significantly depressed the eNOS mRNA in pathological concentration （0.1 mmol/L） and toxic concentration（5.0 mmol/L） compared with the physiological concentration （0.01 mmol/L）. Conclusion：Hcy in higher concentration may lead to the endothelial dysfunction by depressing eNOS and reducing the production of NO.

关 键 词：半胱氨酸 一氧化氮合酶 内皮 血管 细胞 培养的 聚合酶链反应 

分 类 号：R972.6[医药卫生—药品] R541.4[医药卫生—药学]