颈交感阻滞对烧伤早期小鼠各器官IFIT1表达的影响  

Effect of cervical sympathetic block on Interferon-induced protein IFIT1 levels in organs of burned mice during early stage

作　　者：巩继平[1,2] 陈玉培[2] 郭晓姝[3] 粟永萍[1] 

机构地区：[1]第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆400038 [2]重庆医科大学第二临床医学院麻醉科,重庆400010 [3]重庆医科大学基础医学院生理学教研室,重庆400016

出　　处：《第四军医大学学报》2009年第6期485-488,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金海外学者合作研究项目(30328025);创伤;烧伤与复合伤国家重点实验室开放课题(国家"973"项目)(2005CB522605)

摘　　要：目的：观察小鼠心、肝、脾、肺、肾等器官干扰素诱导蛋白IFIT1（Interferon—induced protein with tetratfieopetide repeats1）的表达情况，探讨颈交感神经阻滞（cervical sympathetic block，SB）对烧伤后早期小鼠各器官IFIT1表达状况的影响．方法：将雄性昆明小鼠18只随机分为正常对照组、烧伤组和颈交感神经阻滞（SB）治疗组、烧伤组和SB治疗组采用体表总面积（Total body surface area，TBSA）15％-20％Ⅲ度小鼠烧伤模型，SB治疗组于烧伤后即刻进行双侧颈交感神经阻滞，各组分别于4h后取心、肝、脾、肺、肾组织，提取RNA，以RT—PCR检测IFIT1表达情况．结果：RT—PCR显示IFIT1在各组的各器官均有表达，并存在差异．烧伤组IFIT1在心、肝、脾表达低于正常组，同时在肺、肾表达高于正常组，差异显著（P〈0．01）．SB治疗组IFIT1的表达均低于正常组（P〈0．01）；同时，SB治疗组IFIT1在心、肝、肺、肾的表达也低于烧伤组（P〈0．01）．另外，在各组内的不同器官的IFIT1表达量存在差异（P〈0．05）．结论：烧伤后早期小鼠各器官IFIT1表达有不同的变化，可能与不同器官在烧伤应激反应过程中损伤程度不同有关．SB可显著降低各器官IFIT1的表达．AIM： To observe the expression of IFIT1 （Interferon- induced protein with tetratricopetide repeats 1 ） in the heart, liver, spleen, lungs, kidneys of mice and to detect the effect of cervical sympathetic block （SB） on IFIT1 of organs during the early stage in burned mice. METHOD： Eighteen male Kunming mice were divided randomly into control group, burned group and SB group. Burned group and SB group were inflicted with 15% -20% TBSA （Total body surface area） full thickness burn injury and SB group was treated immediately by SB. Tissues of the heart, liver, spleen, lungs and kidneys were isolated 4 h post-burn. The RNA expression level of IFIT1 was measured by RT-PCR. RESULTS ： The RNA expression levels of IFIT1 in burned group decreased significantly in the heart, liver and spleen and significantly increased in the lungs and kidneys compared with those in control group （P 〈 0.01 ）. The RNA expression levels of IFIT1 in SB group decreased significantly in all the organs examined compared with those in control group（P 〈 0.01 ） and decreased significantly in the heart, liver, lungs and kidneys compared with those in burned group（P 〈0.01 ）. Difference was also found in the RNA expression levels of IFIT1 between the differenct organs examined in each group （ P 〈 0.05 ）. CONCLUSION ： In organs of burned mice during early stage, the changes of RNA expression level of IFIT1 vary with different organs, which may be related to the different degrees of organ injury during stress reaction process. SB can significantly decrease the expression of IFIT1 in organs involved.

关 键 词：IFIT1 颈丛 自主神经传导阻滞 烧伤 

分 类 号：R392.11[医药卫生—免疫学]