3种囊腔人工皱缩技术在小鼠扩张期囊胚玻璃化冷冻中的比较研究  

Comparison of 3 Techniques for Artificial Shrinkage of Blastocoelic Cavity Before Vitrification of Expanded Mouse Blastocysts

作　　者：张宁媛[1] 孙海翔[1] 胡娅莉[1] 徐志鹏[1] 陈林君[1] 招霞[1] 

机构地区：[1]南京大学医学院附属鼓楼医院生殖医学中心,江苏南京210008

出　　处：《中华男科学杂志》2009年第3期228-231,共4页National Journal of Andrology

基　　金：江苏省自然科学基金(BK2004095)~~

摘　　要：目的:比较3种囊腔人工皱缩技术在小鼠扩张期囊胚玻璃化冷冻中的应用,以其建立一种有效的扩张期囊胚玻璃化冷冻预处理方法。方法:扩张期囊胚玻璃化冷冻前分别采用注射针抽吸、拉细管吹打、激光打孔方法预先人工皱缩囊腔,分别与未处理囊腔组比较复苏后囊胚的存活率和孵出率。结果:应用注射针抽吸、拉细管吹打、激光打孔预先处理囊腔,囊胚复苏后的存活率分别72.9%、72.0%、94.0%,显著高于未处理组(40.0%,P<0.05)。囊胚复苏后的囊胚孵出率分别64.6%、32.0%、62.0%,显著高于未处理组(16.0%,P<0.0.5)。结论:预先人工皱缩囊腔,对小鼠扩张期囊胚玻璃化冷冻是一种行之有效的预处理技术,尤以注射针抽吸和激光打孔法为佳。Objective ： To establish an effective pretreatment method for the vitrification of expanded mouse blastocysts by comparing 3 techniques for the artificial shrinkage of the blastocoelic cavity. Methods： The blastoeoelic cavity was artificially shrunk by microneedle aspiration, pipetting or laser drilling prior to the vitrification of the expanded blastocysts. The rates of survival and hatching achieved with the three techniques were compared with those of the non-shrinkage group. Results： The rates of survival were 72.9, 72.0 and 94.0% , and those of hatching were 64.6, 32.0 and 62.0% in the three shrinkage greups, obviously higher than in the nonshrinkage group （40.0 and 16.0% ）. Conclusion： Artificial shrinkage of the blastocoelic cavity was an effective pretreatment technique for the vitrification of expanded mouse blastocysts, especially by micro-needle aspiration and laser drilling.

关 键 词：囊胚 玻璃化冷冻 人工皱缩 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]