地不容总生物碱提取工艺研究  

Studies on Extraction Process for Total Alkaloids in Stephania epigaea

作　　者：李兴[1] 何凤发[1] 张建佳[1] 

机构地区：[1]西南大学农学与生物科技学院,重庆400716

出　　处：《西南大学学报（自然科学版）》2009年第1期127-131,共5页Journal of Southwest University(Natural Science Edition)

摘　　要：通过对地不容总生物碱的不同提取方法进行比较,从而确定最佳提取工艺.进一步采用正交设计法,以总生物碱提取率为考察指标,通过对乙醇浓度、溶媒比、超声波提取时间、回流时间4个因素进行考察,确定最佳提取工艺为:75%乙醇为提取溶剂,乙醇与地不容的溶媒比为12,先超声提取60min,再回流提取1h,共回流提取2次为地不容总生物碱提取的最佳条件.本工艺简单合理,提取率高,具有良好的可行性.The optimum extraction conditions for extracting total alkaloids from Stephania epigaea were studied in an orthogonal experiment, in which 4 factors （alcohol concentration, ethanol： S. epigaea ratio,ultrasonic extraction time and reflux time） were examined, with the extraction rate of total alkaloids as the final indicator. The optimum extraction technology was identified as： extraction solvent, 75 percent etha-nol; ethanol： S. epigaea ratio, 12; ultrasonic extraction time, 60 mim; and two refluxes, 1 h for each time. The process proved to be simple and reasonable, with high extraction rate and good feasibility.

关 键 词：地不容 总生物碱 超声波提取法 正交试验法 

分 类 号：R931.4[医药卫生—生药学]