纳米脂质体槲皮素诱导人食管癌癌干样细胞凋亡的研究  

Human esophageal cancer stem like cell apoptosis induced by nano-liposomal quercetin

作　　者：裴迎新[1] 李金萍[1] 郑乃刚[2,3] 宫璀璀[2,4] 吴景兰[2] 

机构地区：[1]郑州大学公共卫生学院,河南郑州450001 [2]郑州大学分子细胞生物学研究中心 [3]郑州大学基础医学院 [4]中国人民解放军第153医院

出　　处：《山东医药》2008年第43期14-16,共3页Shandong Medical Journal

摘　　要：目的检测纳米脂质体槲皮素对人食管癌癌干样细胞凋亡的诱导作用,并探讨其机制。方法以氯仿-DMSO预溶的脂质体槲皮素经负压旋转蒸发、超声破碎及80nm过滤制备纳米脂质体槲皮素。将其作用于人食管癌细胞系Eca 109/9706细胞的生长抑制率(MTT法),及作用后食管癌细胞中羟自由基(OH.)、NO、PTEN、NF-κB及Caspase-3的表达(免疫组化法)及凋亡率(采用TUNEL法)。检测Eca 109/9706细胞中CD133、MDR1的单/双免疫酶标及单、双荧光标记的复合率。结果纳米脂质体槲皮素处理的Eca 109/9706细胞的生长抑制率、OH.、NO、PTEN、NF-κB及Caspase-3表达和凋亡率均显著高于对照组(P<0.01)。Eca 109/9706细胞的的单免疫荧光/单免疫酶标率各为80%及70%,双免疫荧光/双免疫酶标的复合率为30%～43%。结论纳米脂质体槲皮素可诱导Eca 109/9706细胞产生氧化应激反应并激活PTEN、NF-κB的转导途径,最终通过Caspase-3诱导细胞凋亡;CD1+33、MDR1+可作为癌干样细胞的标志。Objective To detect the induction effect of nano-lipsomal quereetin to human esophageal carcinoma stem llke cell apoptosis and explore the mechanism. Methods The nano-liposomal quercetin was prepared by revolutionary evaporaton under negative pressure, ultrasound wave breakage and 80 nm filtering of nanoliposomal quercetin pre-dissolved in chloroform-DMSO. The growth-inhibition rate, free radical production and NO reactivity, CD^＋133MDR1^＋ in double immu-no-fluorescence/enzyme labeling rate, PTEN-IR, NF-κB-IR and Caspase 3-IR（immunoreactivity） and apoptotic rate were detected in Eca-109/9706 cancer cells. Results The growth-inhibition rate, free radical production, NO reactivity, PTEN-IR, NF-κB-IR, Caspase 3-IR and apoptotic rate were all higher in the nano-liposomal quercetin treated cancer cell group than that in the control cell group. The immuno-fluorescence/enzyme labeling rate was 80% （CD^＋133）and 70% （ MDR1^＋）, CD^＋133 MDR1^＋ in double immuno-fluorescence/enzyme labeling rate was 30%-43%. Conclusions The nano-liposomal quercetin cinhibite apoptotis in human esophageal cancer stem like cells through ROS stress and activated PTEN-IR, NF-κB-IR, Caspase 3-IR cascade pathway; and CD^＋133 ,MDR1^＋ may be used as a marker for cancer stem like cells of Eca-109/9706 cells.

关 键 词：癌干样细胞 细胞凋亡 纳米脂质体槲皮素 食管肿瘤 

分 类 号：R513[医药卫生—内科学]