不同移植方法对脑胶质瘤大鼠骨髓间充质干细胞迁移能力的影响  

Effect of transportation method on the bone marrow-derived mesenchymal stem cells migration in glioma rats

作　　者：高志强[1] 程鹏[2] 刘云会[1] 

机构地区：[1]中国医科大学附属盛京医院,辽宁沈阳110004 [2]中国医科大学附属第一医院

出　　处：《山东医药》2008年第43期21-22,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30700861);辽宁省自然科学基金资助项目(20052102)

摘　　要：目的观察移植方法对脑质瘤大鼠骨髓间充质干细胞(BMSCs)迁移能力的影响。方法立体定向法建立大鼠C6脑胶质瘤模型。A组大鼠用小剂量缓激肽后经荷瘤侧颈内动脉灌注BMSCs组,B组经荷瘤侧颈内动脉灌注尾静脉灌注组,每组6只。免疫组化染色显示MSCs,观察其在正常脑组织和胶质瘤组织中的数量和分布规律。结果三组均可见BMSCs在胶质瘤组织中广泛分布,A组明显多于B、C组(P<0.01)。BMSCs主要分布于肿瘤内部,在肿瘤与正常脑组织的交界处亦有分布。结论小剂量缓激肽有助于BMSCs进入胶质瘤组织。Objective To observe effect of transportation method on the bone marrow-derived mesenchymal stem cells （BMSCs） migration in glima rats. Methods The rat C6 glioma model was established by stereotactie procedure. In group A, BMSCs were injected into internal carotid artery in thetumor side after infusing low dose bradykinin; In group B the BMSCs were injected into internal carotid artery in the tumor side without bradykinin. In Group C the BMSCs were injected into the tail vein. Immunohistochemical methods were used to study the quantity and distribution of MSCs in glioma tissue and the normal cerebral tissue around. Results BMSCs could be seen extensively in the range of glioma tissue in each group ,there were much more BMSCs we could see in group A than that in other groups（P〈0.01）. Most of BMSCs scatterd in the region of glioma. In the juncture area between glioma and normal brain, we could also find BMSCs. Conclusion Low dose of bradykinin is beneficial to BMSCs to migrate into C6 glioma.

关 键 词：颅内肿瘤 神经胶质瘤 骨髓间充质干细胞 移植方法 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]