白桦抗坏血酸过氧化物酶(APX)基因克隆及表达分析  

Cloning and Expression Analysis of An APX Gene from Betula platyphylla

作　　者：王超[1] 杨传平[1] 王玉成[1] 

机构地区：[1]林木遗传育种与生物技术教育部重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2009年第3期79-81,88,共4页Journal of Northeast Forestry University

基　　金：黑龙江省自然科学基金项目(C200803)资助

摘　　要：从白桦(Betula platyphylla Suk.)cDNA文库中获得一个抗坏血酸过氧化物酶(APX)基因全长cDNA序列,该序列去除polyA后长1049bp,其ORF全长750bp,编码250个氨基酸,编码蛋白的相对分子质量为27712.7,理论等电点6.08,保守区分析确定其含有APX蛋白保守序列,属于植物POD超家族。利用实时定量RT-PCR进一步分析白桦苗木在0.8%NaCl胁迫处理下不同时间点APX基因的表达情况。结果表明,盐胁迫处理诱导了APX基因在白桦叶片中的表达,说明APX基因与植物抗盐相关。An Ascorbate Peroxidase （APX） gene was cloned from a eDNA library of Betula platyphyUa Suk. The full eDNA length of APX is 1049 bp when excluding the polyA tail. The ORF of APX is 750 bp in length, encoding 250 amino acid residues with molecular weight of 27 712.7 D and theoretical isoelectrie point of 6. 08. The analysis of conserved domains proves that the gene contains conserved sequence of APX protein and belongs to a member of plant peroxidase superfamily. The expression of APX gene in response to 0. 8% NaCl was further investigated in B. platyphylla using real time RT-PCR. Results show that the APX can be induced by salt in leaf of B. platyphylla, suggesting that it may play a role in plant salt resistance.

关 键 词：白桦 抗坏血酸过氧化物酶 基因表达 实时定量RT—PCR 

分 类 号：S792.153[农业科学—林木遗传育种] Q781[农业科学—林学]