美托洛尔对脓毒症大鼠血清心肌肌钙蛋白Ⅰ、肿瘤坏死因子α及核因子κB的影响  

Effects of metoprolol on expressions of serum cTnI,TNF-α and myocardial NF-κB in septic rats

作　　者：王晓鹏[1] 牟晓杰[1] 王晋平[1] 陈丽[1] 

机构地区：[1]山西医科大学第二医院麻醉科,太原市030001

出　　处：《临床麻醉学杂志》2009年第3期242-244,共3页Journal of Clinical Anesthesiology

摘　　要：目的探讨美托洛尔对脓毒症大鼠心肌的作用及可能的作用机制。方法选择健康雄性SD大鼠18只,随机均分为三组:盲肠结扎加穿孔(CLP)组、美托洛尔干预(M)组和假手术(C)组。M组于CLP后2h,经尾静脉给予美托洛尔0.05mg冲击量,继以0.2mg.kg-1.h-1至CLP5h;C组开腹后不做任何处理。用酶联免疫吸附试验(ELISA)检测血清心肌肌钙蛋白I(cTnI)和肿瘤坏死因子α(TNF-α),同时心肌组织用免疫组化半定量法观察核因子κB(NF-κB)的变化。结果M组、C组血清cTnI、TNF-α及心肌NF-κB均低于CLP组(P<0.05);M组血清cTnI与血清TNF-α、心肌NF-κB之间均呈正相关。结论美托洛尔有保护脓毒症大鼠心肌损伤的作用,其机制与抑制TNF-α及NF-κB的表达有关。Objective To investigate the effect of metoprolol on the myocardial in the septic rats. Methods Male Sprague-Dawley rats were randomly divided into group C （sham operation group） ; group CLP and grqup M. Cecal was ligated and perforated in group CLP and M. Rats in group M were administrated metoprolol 0.2 mg·kg^-1·h^-1 after 0. 5 mg bolus injection through caudal vein 2 h after CLP until CLP 5 hours. The serum cTnI and TNF—α were measured with ELISA,the myocardial NF-κB was detected by immunohistochemistry. Results The expressions of cTnI, TNF—α and NF-κB were significantly tower in group C and M than those in group CLP （P〈 0.05）. The level of cTnI was positively correlated to that of TNF—α（r=0. 946,P〈0.01） and NF-κB （r=0. 918,P〈0.01）in group M. Conclusion Metoprolol can protect rats from septic myocardial injury,possibly by decreasing the serum TNF—α and myocardial NF-κB. B

关 键 词：脓毒症 美托洛尔 心肌肌钙蛋白Ⅰ 肿瘤坏死因子Α 核因子ΚB 

分 类 号：R459.7[医药卫生—急诊医学]