DOPA在聚乙烯微孔膜上的自聚合及肝素固定化  

SELF-POLYMERIZATION OF DOPA ON POLYETHYLENE POROUS MEMBRANES AND IMMOBILIZATION OF HEPARIN

作　　者：朱利平[1] 徐又一[1] 奚振宇[1] 朱宝库[1] 

机构地区：[1]浙江大学高分子科学研究所大分子合成与功能构造教育部重点实验室,杭州310027

出　　处：《高分子学报》2009年第4期394-397,共4页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号50803054);国家重点基础研究发展计划(973计划;项目号2007C13623402)资助项目

摘　　要：在溶液条件下,左旋3,4-二羟基苯丙氨酸(L-DOPA,或L-多巴)能在固体材料表面自发聚合,形成强力附着于材料表面的poly(DOPA)层.基于L-DOPA的这一特性,对聚乙烯(PE)微孔膜进行了表面改性,得到了PE/poly(DOPA)复合膜,并进一步通过poly(DOPA)层上的反应性基团将肝素共价固载在PE微孔膜上.X光电子能谱(XPS)分析结果证实了poly(DOPA)和肝素的固定,接触角测量数据表明膜的亲水性得到了显著提高.A new and convenient approach for surface immobilization of heparin onto PE porous membranes is reported.3,4-dihydroxy-L-phenylalanine （L-DOPA）, a catecholic amino acid, is an important component of mussel adhesive proteins （MAPs）. Based on the strong adhesive behavior of poly（DOPA） on solid surface, hydrophobie PE porous membranes was surface-modified by self-polymerization of L-DOPA in aqueous solution. Subsequently, heparin was immobilized covalently onto PE/poly （DOPA） composite membranes by the coupling between heparin and poly （DOPA） layer. The immobilization of poly （DOPA） and heparin was confirmed by X-ray photoelectron spectroscopy （XPS）. The results of water contact angle measurements showed that the hydrophilieity of PE membranes was significantly improved by the introduction of poly（DOPA） and heparin. The effects of poly（DOPA） and heparin on membrane surface morphologies were also investigated by scanning electron microscopy （SEM）. Despite the decreased pore amount and pore size on membrane surface after poly（DOPA） and heparin immobilization, the improved hydrophilicity would be beneficial to promote permeability and biocompability of PE membranes.

关 键 词：多巴 聚乙烯微孔膜 自聚合 肝素固定 

分 类 号：R318.08[医药卫生—生物医学工程]