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作 者:唐全[1] 曹帮华[1] 刘凤[1] 贾波[1] 曹玉翠[1]
出 处:《西南大学学报(自然科学版)》2009年第3期94-99,共6页Journal of Southwest University(Natural Science Edition)
基 金:山东省农业良种工程项目计划资助项目(鲁科农字【2006】90号)
摘 要:采集悬铃木不同年龄母树的一年生硬枝进行扦插试验,扦插前用质量分数为0.01和0.05的-αNAA处理2 h,清水为对照,测定POD、PPO、IAAO活力及C/N,探讨其与插穗生根的关系.结果表明:悬铃木硬枝扦插中存在年龄效应;-αNAA能够扭转这种年龄效应、提高多年生插穗的生根能力;随着母树年龄的增加,硬枝插穗中的POD、PPO、IAAO活力及可溶性糖含量、C/N值均呈下降趋势,插穗内可溶性糖含量、C/N值与插穗生根率正相关;三种氧化酶活性呈现规律性变化,不同处理间和各生根阶段3种酶活性呈现差异性,说明在悬铃木扦插生根过程中,不定根的发生发展与3种酶活性有密切联系.A cutting experiment was done with hardwood cuttings from mother plants of different ages and treated with different concentrations (100 and 500mg/L) of α-NAA for two hours. The activities of POD, PPO and IAAO and the C/N ratio were determined, and their relationship with rooting rate was discussed. The results showed that an age-effect existed in the rooting of hardwood-cuttings of P. orientali; α-NAA could offset the "age-effect" and promote rooting rate of aging mother plants; the activities of POD, PPO and IAAO, soluble sugar content and C/N in the hardwood decreased with increasing age of the mother plant; soluble sugar content and C/N were in significant positive correlation with rooting rate; differences were observed among all the treatments and rooting stages. The above results suggested that the activity of the 3 oxidases studied were closely related to the rooting process of the cuttings of P. orientali.
关 键 词:悬铃木 年龄效应 硬枝扦插 生根 氧化酶 营养物质
分 类 号:S72[农业科学—林木遗传育种]
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