121株甲型副伤寒病原菌的噬菌体型和脉冲场凝胶电泳型  

Phagocyte types and pulsed field gel electrophoresis patterns of 121 strains of Salmonella enterica serotype Paratyphi A isolates

作　　者：曾丽萍[1] 姚云波[1] 刘红雁[1] 杨汝松[1] 王树坤[1] 吴强[1] 李顺祥[1] 

机构地区：[1]玉溪市疾病预防控制中心,云南玉溪653100

出　　处：《中国微生态学杂志》2009年第3期239-241,共3页Chinese Journal of Microecology

摘　　要：目的认识甲型副伤寒疫病区甲型副伤寒沙门菌的噬菌体型和脉冲场凝胶电泳(PFGE)型,确定噬菌体型和PFGE型之间的关系以及菌型的分布和流行率。方法采用沙门菌组合噬菌体和SpeI、XbaI消化染色体DNA的PFGE对来自玉溪市7县(区)的121株甲型副伤寒菌进行分型。结果121株菌存在4个完全噬菌体型或1个噬菌体型;用SpeI或XbaI消化产物分别得出以SpeI01、SpeI02或XbaI01占优势的5种或4种PFGE型,SpeI01型和SpeI02型分别占37.2%和57.9%,XbaI01型占95.1%。结论121株菌的噬菌体型与PFGE型之间无一致性联系,PFGE型的SpeI01和SpeI02或XbaI01是玉溪地区的主要流行型,采用SpeI和XbaI的PFGE是鉴别甲型副伤寒菌流行克隆的一项有用技术。Objective To understand the phagocyte types and pulsed field gel electrophoresis （PFGE） patterns of Salmonella enterica serotype Paratyphi A isolates from Paratyphoid fever A epidemic area, and confirm the links between the phagocyte types and PFGE patterns as well as the distribution and prevalence of the isolates. Method A total of 121 isolates were studied using Salmonella combined phagocytes and PFGE analysis following digestion of chromosomal DNA with SpeI and XbaI. Result There were 4 complete phagocyte types or 1 phagocyte type among the 121 isolates. The SpeI and XbaI digestions of the 121 isolates gave five or four different PFGE patterns in which the SpeI01 and SpeI02 （ or XbaI01 ） epidemic patterns were predominant respectively. SpeI01 and SpeI02 accounted for 37. 2% （45/121） and 57. 9% （70/121） of the isolates, respectively,or XbaI01 accounted for 95.1% （ 115/121 ） of the isolates. Conclusion No links were observed between the phagocyte types and PFGE patterns. PFGE patterns SpeI01 and SpeI02 （or XbaI01 ）, the main clones of the epidemics, are highly prevalent in Yuxi. PFGE with SpeI and XbaI is a useful technique to differentiate SPA from different geographical regions, and can be useful for epidemiological routine investigation of PA.

关 键 词：副伤寒 甲型副伤寒沙门菌 噬菌体型 PFGE型 

分 类 号：R378[医药卫生—病原生物学]