水稻花培苗试管保存技术的研究  

Studies on the Test Tube Restoring Technique of Anther Cultured Seedlings in Rice

作　　者：王永新 刘亚琴 张启星 刘国庆 

机构地区：[1]河北省稻作研究所,唐海063200

出　　处：《河北农业大学学报》1998年第2期6-9,共4页Journal of Hebei Agricultural University

基　　金：国家"八五"攻关研究课题

摘　　要：以水稻杂交F1代花药培养所获得的幼苗为材料，研究了花培苗在最低量生长条件下长时间保存于试管的适宜条件。花培苗在含Bi－MET5．0mg／L和6－BA2．0mg／L的培养基上，15～20℃的弱光条件下试管培养不需要中途转管，保存了8个月。试管苗表现为叶片宽厚，茎秆粗壮，叶色深绿，发达。入土移栽手续简便．移栽成活率达85％。The suitable conditions under which the anther cultured seedlings could be restored in test-tube for a long term in minimum growing conditions were studied by using the anther cultured seedling which came from the hybrid F1. The seedling which cultured on the medium containing Bi - MET 5. 0mg/L and 6 - BA 2. 0mg/L under 15 ~ 2℃ and dimlight, could be restored for as long as 8 months in the tube without transplanting. The leaves of seedlings were thick and wide, strong in stems , and big in root systern. The process of transplanting them into soil was simplfied and the survival ratio could reach 85 %.

关 键 词：水稻 花培苗 最低量生长 试管保存 

分 类 号：S511.035.3[农业科学—作物学]