野大麦幼根和幼叶悬浮细胞系的原生质体培养  

Protoplast Culture from Suspension Cell Line of Young Root and Leaf of Wild Barley ( Hordeum brevisubulatum (Trin.) Link)

作　　者：程肖蕊[1] 张亚兰[1] 杨松涛[1] 李彦舫[1] 

机构地区：[1]解放军农牧大学生物教研室

出　　处：《西北农业大学学报》1998年第2期22-26,共5页Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)

基　　金：总后军需部课题资助

摘　　要：以野大麦（Ｈｏｒｄｅｕｍｂｒｅｖｉｓｕｂｕｌａｔｕｍ（Ｔｒｉｎ．）Ｌｉｎｋ）的幼根、幼叶为外植体，诱导愈伤组织，建立悬浮细胞系。利用胚性悬浮细胞系进行质壁分离处理再游离原生质体，在不同的光照条件下进行原生质体培养，并由原生质体再生出小细胞团。In this study,young roots and young leaves of wild barley ( Hordeum brevisubulatum (Trin.) Link) were used as explants to induce calli.Through several subcultures,embryogenic calli were selected and adopted to establish suspension cell lines.Protoplasts were isolated from embryogenic suspension cell line after plasmolysis treatment and cultured under different light conditions.Cell mini clusters regenerated from protoplasts.

关 键 词：野大麦 原生质体 细胞分裂 育种 

分 类 号：S512.303.5[农业科学—作物学]