17β-雌二醇对氧糖剥夺神经元的保护作用  

17β-estradiol pretreatment protects rat cortical neurons injured by oxygen-glucose deprivation in vitro

作　　者：杨丽娟[1,2] 邬力祥[1] 李伟[3] 贺芳[1] 张琰[1] 黄柏胜[1] 

机构地区：[1]中南大学湘雅医学院生理学系,长沙410078 [2]滨州医学院生理教研室,山东滨州256603 [3]滨州医学院附属医院胸心外科,山东滨州256603

出　　处：《第三军医大学学报》2009年第7期585-588,共4页Journal of Third Military Medical University

基　　金：教育部博士点基金(20050533062)~~

摘　　要：目的研究17β-雌二醇(17β-estradiol,E2)对氧糖剥夺大鼠皮层神经元的保护作用。方法体外培养鼠脑皮层神经元,建立缺血缺氧模型。利用MTT代谢法、流式细胞术和Hoechst33342荧光染色观察E2(10-8mol/L)对缺氧损伤皮层神经元细胞活力和凋亡的影响;硫代巴比妥酸法测定丙二醛(MDA)含量,黄嘌呤氧化酶法测超氧化物歧化酶(SOD)活力变化。结果氧糖剥夺后神经元细胞活力显著降低,凋亡率升高,MDA生成增多,SOD的活力下降,与空白对照组相比有统计学差异(P<0.05);E2预处理后细胞活力升高、凋亡率下降、MDA生成减少、SOD的活性升高,与氧糖剥夺组相比有统计学差异(P<0.01)。结论E2对氧糖剥夺神经元具有保护作用,其作用可能与SOD活性提高拮抗氧自由基对细胞的损伤有关。Objective To explore the effect of 17β-estradiol on rat cortical neurons injured by oxygenglucose deprivation （OGD）. Methods Primary cortical neurons were isolated from neonatal rats and identified with NSE staining, then cultured for 10 d under normal condition. To establish OGD injury, the culture medium was replaced by glucose-free medium, and cells were transferred to a humidified incubation chamber flushed by a gas mixture of 95% N2 and 5% CO2 at 37 ℃ for 2 h. To explore the influence of 17β-estradiol on neurons under OGD for 2 h condition, the cells were pretreated with 10-8mol/L 17β-estradiol 24 h prior to OGD. The cell viability were evaluated by 3- （ 4,5-Dimethylthiazol-2-yl ） -2,5-diphenyltetrazolium bromide （MTF） assay, and the percentage of apoptotic cells was tested by flow cytometry and Hoechst 33342 staining. The contents of MDA and the activities of SOD were evaluated by biochemical methods. Results The results showed that OGD injury significantly decreased the cell viability and the activities of SOD, increased the percentage of apoptotic cells and the content of MDA （P 〈 0.05）. Pretreament of 10^-8mol/L 17β-estradiol increased the cell viability and the activities of SOD, decreased the contents of MDA and the percentage of apoptotic cells in cortical neurons exposure to OGD （P 〈 0.01 ）. Conclusion 17β-estradiol has the neuroprotective effect on cortical neurons after OGD injury. This effect of 17β-estradiol may be mediated by eliminating the free radicals by increasing the activities of antioxidative enzymes and thus inhibit the oxidative damages caused by OGD.

关 键 词：17Β-雌二醇 神经元 氧 葡萄糖 自由基 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]