高渗氯化钠羟乙基淀粉40注射液对大鼠全身高温模型血脑屏障通透性的影响  

Effects of hypertonic sodium chloride hydroxyethyl strach 40 injection on the permeability of blood brain barrier in the whole-body hyperthermic rat＇s model

作　　者：占丽芳[1] 侯小敏[1] 刘友坦[1] 赵振龙[1] 古妙宁[1] 

机构地区：[1]南方医科大学附属南方医院麻醉科,广州510515

出　　处：《国际麻醉学与复苏杂志》2009年第2期120-123,共4页International Journal of Anesthesiology and Resuscitation

基　　金：深圳市科技局项目（200802029）

摘　　要：目的观察高渗氯化钠羟乙基淀粉40注射液对大鼠全身高温模型血脑屏障（blood brain barrier，BBB）通透性的影响并探讨其作用机制。方法成年雄性SD大鼠60只，随机分为正常对照组（C组），高温组（HT组），高温林格组（RL组），高温羟乙基淀粉林格组（HRL组），高温高渗氯化钠羟乙基淀粉组（HSH组），C组大鼠置于（25～26）℃的环境中4h，其余各组大鼠置于36℃具备生物氧供给的加温舱中3h达到肛温（41～42）℃，加温开始30min内根据实验分组输入相应的液体，3h后出舱在室温下降温，加温期间监测肛温、平均动脉压（mean arterial blood pressure，MAP）和动脉血气。采用伊文思兰（evans blue，EB）法检测大鼠血脑屏障的通透性，干湿法检测大鼠脑组织的百分含水量。结果与C组相比，其他各组大鼠的脑百分含水量、肛温和EB值均有显著的升高（P〈0．01），但HSH组升高值较其余3组为低（P〈0．01）。各实验组大鼠的MAP、pH、PaO2、PaCO2、Hct与C组相比差异有统计学意义（P〈0．05）。Na^＋、K^＋在HT组和HSH组明显升高（P〈0．01），其余各组差异无统计学意义。结论全身高温可以导致大鼠BBB通透性增加，高渗氯化钠羟乙基淀粉40注射液能够减轻全身高温对大鼠BBB的损害。Objective To investigate the effects of hypertonic sodium chloride hydroxyethyl strach 40 injection（HSH）on the permeability of blood brain barrier （BBB）in the whole-body hyperthermic （WBH） rat＇s model, and to explore the mechanism. Methods 60 adult male Sprauge-Dawley rats（220-250）g were randomized into 5 groups. Twelve rats were housed at a room with 25℃-26℃ temperature for 4h as control （C group）. Other rats were exposed to WBH （36℃） to increase the rectal temperature to 41℃-42℃ for 3 h, thereafter were moved out of the heating container to let the temperature recover down for 1 h. At WBH beginning, nothing （HT group）, Ringer＇s solution （RL group）, Haes ＋ Ringer＇s solution （HRL group）, or HSH （HSH group）was administered intravenously within 30 rain（n= 12）. In the end of experiment, BBB permeability and brain water content were measured. Results After 3 h WBH, BBB and water content in WBH groups were significantly higher than those in group C （P〈0.01）, With the lowest increasing extent in group HSH among test rats. MAP, blood pH, PaO2, PaCO2, and Hct significantly changed in test groups （P〈0.05）, and plasma Na^＋ and K^＋ concentration markedly increased in group HT and group HSH （P〈0.01）. Conclusion HSH infusion may attenuate WBH-induced high BBB permeability in rats.

关 键 词：全身高温 脑水肿 伊文思兰 血脑屏障 高渗氯化钠羟乙基淀粉40注射液 

分 类 号：R96[医药卫生—药理学]