鞘内注射地塞米松对背根节压迫大鼠镇痛作用的行为学观察  

Analgesic effects of intrathecal dexamethasone injection on chronic dorsal root ganglion compression-induced pain in mouse

作　　者：张兢[1] 顾小萍[2] 郭鑫[2] 马正良[2] 张娟[2] 史长喜[2] 

机构地区：[1]徐州医学院江苏省麻醉医学研究所&江苏省麻醉学重点实验室,221002 [2]南京大学医学院附属鼓楼医院麻醉科

出　　处：《国际麻醉学与复苏杂志》2009年第2期134-138,共5页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金资助项目（30872439/c160202）;卫生部课题资助项目（WKJ2006-2-8）;江苏省科教兴卫工程重点人才课题（RC2007012）;江苏省教育厅开放课题（KJS06001）

摘　　要：目的在大鼠慢性背根神经节压迫模型中观察鞘内注射地塞米松的镇痛效果。方法制作慢性背根神经节压迫模型（CCD组），同时制作不给予背根神经节压迫的假手术模型（sham组）。所有的实验大鼠在术前3d，术后第3、4、7、10、13、14、15天检测痛行为学指标，包括机械痛缩足阈值（paw with drawal mechanical threshold，PWMT）和热痛缩足潜伏期（paw withdrawal thermal latency，PWTL）。CCD模型组分为10％DWSO对照组（C组）、早期治疗组（ET组）、后期治疗组（LT组）和二次治疗组（TT组）。10％DWSO对照组在术后第3天和第13天鞘内给予10％DWSO，早期治疗组于术后第3天分别给予地塞米松，第13天给予10％DWSO。后期治疗组于术后第3天给予10％DWSO，第13天给予地塞米松，二次治疗组术后第3、13天均给予地塞米松。结果术后3d空白对照组PWMT（2．67±1．03）较sham组PWMT（11．33±2．94）和术前基础值PWMT（13．33±2．58，13．33±2．58，13．33±2．58）有显著降低（P〈0．05）、C组PWTL（8．82±1．82）较sham组（22．14±2．34）和基础值（22．52±1．94，21．59±2．49，22．46±1．52）明显缩短（P〈0．05）。鞘内给药之后ET组和TT组PWMT增高（P〈0．05），PWTL延长（P〈0．05）；但LT组PWTL、PWMT无明显变化（P〉0．05）。结论鞘内注射地塞米松可以有效改善慢性背根神经节压迫引起的痛行为学反应，但具有时间依赖性。Objective To investigate the analgesic effects of intrathecal dexamethasone injection on pain induced by chronic compression of dorsal root ganglion in mouse. Methods The test model（CCD group） and sham model（sham group） of chronic dorsal root ganglion compression were established. Pain ethology indexes such as paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） were assessed on 3 d before and 3 d, 4 d, 7 d, 10 d, 13 d, 14 d and 15 d after operation. The mice in group CCD were subdivided randomly into solventia group （C group）, early treatment group（ET group）, later treatment group （LT group）and twice treatment group （TT group）. Vehicle （10 % DMSO）and dexamethasone were intrathecally injected at both 3 and 13 postoperative day in groups sham, C and group TT, respectively. Dexamethasone and vehicle were injected at 3 and 13 postoperative day, respectively in group ET. Vehicle and dexamethasone were injected at 3 and 13 postoperative day, respectively in group LT. Results Compared to group sham and preoperative level, PWMT and PWTL decreased at 3 postoperative day in group C （P〈0.05）. Intrathecal dexamethasone injection significantly increased PWMT and PWTL （P〈0.05） in both groups ET and TT, however with no obvious change in pain ethology indexes in group LT. Conclusion Intrathecal dexamethasone injection alleviates pain behavior induced by chronic dorsal root ganglion compression in mouse in a time dependence manner.

关 键 词：糖皮质激素 神经病理性疼痛 脊髓 

分 类 号：R96[医药卫生—药理学]