高丹草SSR引物设计及分子遗传框架图谱构建  

SSR Primer Designing and Construction of a Genetic Map of Sorghum bicolor×S. sudanense

作　　者：卢杰[1,2] 吕媛媛[1] 李杰勤[1] 詹秋文[1] 王敏[2] 

机构地区：[1]安徽科技学院,安徽凤阳233100 [2]安徽农业大学,安徽合肥230036

出　　处：《中国草地学报》2009年第2期28-33,共6页Chinese Journal of Grassland

基　　金：科技部农业科技成果转化资金项目(No:2008GB2C300125);国家科技支撑计划项目(No:2008BADB3B3B10);安徽省“十一五”科技攻关计划重点项目(No:06013104B);安徽省高校学科拔尖人才基金(No:教秘人〔2005〕79号);安徽省自然科学基金资助项目(No:01041102)

摘　　要：利用高粱EST序列和BAC克隆,以Primer 5.0程序设计了10对高丹草SSR引物,结果发现有3对引物在皖草2号高丹草的亲本间表现多态性,其中2对来自BAC的引物可用于遗传连锁图谱构建。除以上10对SSR引物外,再选取已公布的245对高粱SSR引物,以180个皖草2号(TX623A×S722)的F2代单株作为构图群体,构建了一个包括4个连锁群、33个SSR标记的高丹草遗传图谱,图谱覆盖基因组长度458.5cM,平均图距13.8cM。Ten SSR primer pairs derived from EST and BAC in sorghum were developed using the software Primer 5.0 and 3 of them were polymorphic between Sorghum bicolor TX623A and S. sudanense S722, 2 of them were used for construction of genetic map. A genetic map of Sorghum bicolor × S. sudanense was constructed based on 10 SSR primer pairs developed and 245 publicly available SSR primer pairs for sorghum with a population of 180 F2 individuals derived from a cross between TX623A and S722. The map consisted of 4 linkage groups and 33 SSR makers. The map covered 458.5cM with average genetic distance of 13.8cM.

关 键 词：高丹草 SSR 引物设计 遗传图谱 构建 

分 类 号：S544[农业科学—作物学] Q943.2[生物学—植物学]