从母女咽拭子样本中检出EV71肠道病毒核酸  

Enterovirus 71 nucleic acid detected from throat swab specimens of a pair of mother-daughter

作　　者：刘丹[1] 王建红[1] 苏豪浩[1] 范玉山[1] 

机构地区：[1]河北省唐山市疾病预防控制中心,河北唐山063000

出　　处：《中国卫生检验杂志》2009年第3期501-502,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:诊断一例儿童手足口病,同时检查患儿母亲是否感染相同的肠道病毒。方法:采集患儿及其母亲的咽拭子标本,采用逆转录聚合酶链反应法(RT-PCR)检测肠道病毒核酸,用琼脂糖凝胶电泳判定结果。结果:患儿及其母亲标本均为EV71阳性,结合临床特征诊断患儿系EV71引起的轻微手足口病,其母亲无临床症状。结论:EV71的家庭内接触传染应该得到关注;在EV71相关的手足口病流行期更要重视疾病监测工作。Objective：To determine whether a child with hand-foot-and-mouth disease（HFMD） is caused by enterovirus 71 infection and the patient′s mother is also tested with the same measure.Methods：The throat swab specimens of a 4-year-old girl with HFMD and her mother were examined with reverse transcription-PCR.Agarose gel electrophoresis was used to determine the amplified products.Results：The mother-daughter′s throat swab specimens were all positive for EV 71 nucleic acid.Combining clinical features,the child′s illness diagnosed as mild HFMD due to EV71 infection,and the mother was asymptomatic.Conclusion：Intrafamilal transmission of EV71 should be consided.More careful disease surveillance is needed during EV71-associated HFMD prevalence.

关 键 词：肠道病毒71型 RT-PCR 诊断 

分 类 号：R373.2[医药卫生—病原生物学]