胶乳增强透射比浊法测定糖化血红蛋白的方法学评价  

Evaluation of method of detecting glycosylated hemoglobin A_(1C) in whole blood with latex particle intensified immunity transmission turbidity

作　　者：郑佳音[1] 陈筱菲[1] 陈晓东[1] 林向阳[1] 袁谦[1] 孙禾[1] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000

出　　处：《中国卫生检验杂志》2009年第3期569-570,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:研究胶乳增强透射比浊法测定糖化血红蛋白在临床应用的可靠性。方法:在日立生化分析仪7600上用胶乳增强透射比浊法测定糖化血红蛋白,对其批内、日间重复性、回收实验、不同样本前处理方式造成的干扰进行评价,并与乳胶凝集抑制法作相关比较。结果:该方法的批内重复性为0.9%,日间重复性为3.96%;平均回收率分别为105.3%和103.7%。与乳胶凝集抑制法有较好相关性,Y=0.909X+0.28,r=0.974;标本前处理中,不同的溶血时间对糖化血红蛋白的结果无明显干扰。结论:胶乳增强透射比浊法有较好的精密度和准确度,成本低,适合自动化,操作简便,适合临床开展。Objective：To study the reliability of the method of detecting the glycosylated hemoglobin A1c in whole blood with latex particle intensified immunity transmission turbidity.Methods：Used the method on latex particle intensified immunity transmission turbidity to detect the glyeosylated hemoglobin A1C and also evaluated the precision,comparative test and the result of different operation.Results：The inter-CV and the intra-CV for GHb were 0.9% and 3.63%.The average recovery rates were 105.3% and 103.7% respectively.And it had great relativity with latex particle agglutination inhibition.The different operation had little interference.Conclusion：Immunity transmission turbidity with latex particle intensified has a good precision and accuracy.It is a rapid method which can act as a clinical routine method for measuring GHb.

关 键 词：胶乳增强透射比浊法 糖化血红蛋白 

分 类 号：R446.11[医药卫生—诊断学]