哈尔滨地区引起婴幼儿腹泻轮状病毒的分子流行病学特征  

Molecular epidemiologic characteristics of rotaviruses from infants and children with diarrhea in Harbin

作　　者：任莉娜[1] 张炳丽[1] 梁英[1] 怀清杰[1] 刘晓慧[1] 李晓鹏[1] 梁爽[1] 范荣军[1] 

机构地区：[1]哈尔滨市疾病预防控制中心,哈尔滨150056

出　　处：《中国卫生检验杂志》2009年第3期596-599,共4页Chinese Journal of Health Laboratory Technology

基　　金：哈尔滨市科技创新人才研究专项资金项目(2006RFQQSO62)

摘　　要：目的：了解哈尔滨地区引起婴幼儿腹泻轮状病毒（Rotavirus,RV）的发病规律和血清型特征,为轮状病毒疫苗在中国东北地区的应用提供流行病学背景资料。方法：采用酶联免疫吸附实验、巢式逆转录聚合酶链式反应,对2007年1月～12月随机采集哈尔滨市儿童医院3岁以下腹泻住院患儿粪便标本,进行RV抗原检测和血清型分型。结果：247份标本男性143份,女性104份,共检出RV抗原阳性141份（阳性率57.09%）,男性阳性率51.05%（73/143）,女性阳性率65.38%（68/104）;RV腹泻患儿年龄在7～12月龄发病最多为70例（49.65%）;秋冬季（1月、11月、12月）为RV腹泻发病高峰季节,为73例（51.77%）;对70份优势毒株进行G、P血清型分型,G3型30株（42.86%）为主,其次G1型19株（27.14%）,G4型3株,G2型、G9型各1株,混合型8株,未分型8株;P[4]型16株（22.86%）,P[8]型49株（70.00%）,未分型5株;组合型以G3P[8]型21株最多,G1P[8]型16株次之。结论：RV腹泻女性阳性率高于男性阳性率;RV发病年龄以7～12月龄人数最多;本年度G3型是主要流行毒株,组合型G3P[8]为流行优势株。Objective：To characterize the molecular epidemiology of rotavirus infection and the distribution of G and P serotype of rotavirus in Harbin.Application of rotavirus vaccine in northeast area of China provide epidemiologic background information.Methods：247 stool samples,collect from infants and children less than three years of age hospitalized of Harbin Children Hospital in 2007,were tested for rotavirus antigen by ELISA and G,P serotyping by Nested RT-PCR.Results：141（57.09%） rotavirus-positive samples were dected in 247（man 143,women 104） fecal specimens during the 2007.The positive rate of man is 51.05%（73/143）,the positive rate of women is 65.28%（68/104）.A peak age distribution of rotavirus diarrhea was 7 to 12 months old （70/141,49.65%）.Rotavirus infection peaked in winter month（in January,November,December）.Among 70 strains performed with G,P serotypes.Serotype G3 represented 42.86% of the G-typed sample predominating in 70 strains.Serotype G1 was the next most common,representing 27.14% of G-typed samples.3 stains were G4,1 stains was G2,1 stains was G9 and 8 strains were mixed.With P serotypes,16 strains were P[4],49 strains were P[8].21 strains were G3P[8] that the most common combined sterotypes,16 strains were G1P[8] that the next most common combined serotypes.Conclusion：Compared with men,women had a higher positive rate of rotavirus infection in Harbin.A peak age distribution of rotavirus diarrhea was 7 to 12 months old.Rotavirus serotype G3 and combined serotype G3P[8] were prevalent strains during the 2007,in Harbin.

关 键 词：轮状病毒 婴幼儿 腹泻 血清型 G型 P型 

分 类 号：R446.5[医药卫生—诊断学]