扶正解毒含药血清对染镍细胞表达P38MAPK的影响  

Influence of medical serum with effects of nourishing healthy qi and detoxication on expression of P^(38MAPK) in nickel-dyed cells

作　　者：王婷[1] 马继业 吴瑞贤[3] 段舞云[3] 朱玉真[1] 马燕龙[1] 

机构地区：[1]兰州大学中西医结合研究所,甘肃730000 [2]甘肃积石山人民医院 [3]甘肃金川集团公司职工医院

出　　处：《北京中医药大学学报》2009年第2期112-114,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：教育部博士点专项科研基金资助项目(No.2005730031)

摘　　要：目的探讨扶正解毒含药血清对染镍人支气管上皮细胞(16HBE)表达P38丝裂原活化蛋白激酶的影响,阐述镍化合物致癌的分子机制。方法用生理盐水,硫化镍(NiS),NiS+扶正解毒含药血清(低、中、高)剂量来处理16HBE后,用Western-blot法观察磷酸化P38MAPK的表达,处理细胞后提取蛋白,电泳、转膜、封闭、杂交、显影、洗脱。结果硫化镍能明显激活16HBE磷酸化P38MAPK的表达,硫化镍浓度越高,磷酸化P38MAPK的表达值越大,且存在剂量-效应关系,扶正解毒含药血清能明显抑制磷酸化P38MAPK的表达,尤以中、高剂量扶正解毒含药血清为甚,NiS+扶正解毒含药血清(中、高)剂量组与NiS比较抑制了磷酸化P38MAPK的表达(P<0.01)。结论硫化镍能明显激活磷酸化P38MAPK的表达,这可能是镍致癌的分子机制之一;扶正解毒含药血清能明显抑制16HBE磷酸化P38MAPK的表达,对于镍化合物诱发肿瘤的预防及治疗具有重要的意义。Objective To investigate the influence of medical serum with effects of nourishing healthy qi and detoxication on the expression of phosphorylated 38 mitogen-activated protein kinases （p38MAPK） in human nicked-dyed bronchial epithelial （16HBE） cells, and explain the carcinogenic mechanism of nickel compound at the molecular level. Methods The expression of p38MAPK was observed by using Western blotting method after 16HBE cells were dealt with respectively the normal saline water, nickel sulfide （NiS） solution and different concentrations of solution combined NiS and medical serum with effects of nourishing healthy qi and detoxication （in high, medium and low doses）. Afterwards the protein was extracted, given electrophoresis, transmembrane, locking, hybridization, development and elution. Results NiS solution activated significantly the expression of p38MAPK in 16HBE cells, and the higher the concentration of NiS sontution was, the larger the expression value of p3SMAeK was, which showed a relationship of dose-effect. The medical serum inhibited significantly the expression of p38MAPK, especially in high and medium doses. The solution combined NiS and medical serum inhibited the expression of p38MAPK compared with NiS solution （P 〈0.01 ）. Conclusion NiS can activate significantly the expression of p38UAPK in 16HBE cells, which may be the carcinogenic mechanism of nickel at the molecular level. The medical serum with effects of nourishing healthy qi and detoxicating can inhibit significantly the expression of p38MAPK in 16HBE cells, which is important to the prevention and treatment of cancer induced by nickel compound.

关 键 词：扶正解毒含药血清 P38MAPK 染镍细胞 兔 

分 类 号：R285.5[医药卫生—中药学]