3种中药单体成分对猪孤雌激活胚胎体外发育效果的影响  

Effects of Three Kinds of Chinese Medicine Simplex Components on Porcine Parthenogenetic Embryos Development in Vitro

作　　者：徐捷[1] 李冬冬[1] 张志刚[1] 刘笑然[1] 赵曌[1] 赵菊[1] 相荣科[1] 高建明[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《中国畜牧兽医》2009年第3期112-115,共4页China Animal Husbandry & Veterinary Medicine

基　　金：北京市教委资助项目(KM200710020014)

摘　　要：为了建立良好的猪胚胎体外培养体系,以体外成熟培养的卵母细胞为材料,研究中药单体成分川芎嗪(Lig)、小檗碱(BR)和淫羊藿苷(Ica)对猪孤雌激活胚胎体外培养效果的影响。以NCSU-23基础培养液为对照组,采用同一种孤雌激活方案,在培养液NCSU-23中分别添加中药单体成分川芎嗪、小檗碱和淫羊藿苷的小、中、大剂量(Lig1、Lig2、Lig3;BR1、BR2、BR3;Ica1、Ica2、Ica3)为3个试验组,比较了各组猪孤雌激活早期胚胎的发育效果,从而进行中药单体成分最佳浓度的筛选。结果显示:①孤雌激活胚胎体外发育至48h的卵裂率和第7天的囊胚发育率,Lig1组与对照组和Lig2组之间差异显著(P<0.05),与Lig3组差异极显著(P<0.01)。②BR2组的卵裂率与对照组和BR3组之间差异显著(P<0.05),与BR1组无显著差异(P>0.05)。第7天囊胚发育率BR2组显著高于其他各组(P<0.05),其他各组之间无显著差异(P>0.05)。③Ica2组胚胎发育至48h的卵裂率和第7天的囊胚发育率均显著高于其他各组(P<0.05),而其他各组之间无显著差异(P>0.05)。由此可见,在NCSU-23基础液中添加3种中药单体成分最佳浓度均能显著提高猪孤雌胚体外发育效果。In order to based better cultural system of porcine embryos in vitro, the maturated oocytes were selected as the experimental material in this study, to approach the effects of Ligustrazine, Berberine and Icariin on culture of porcine parthenogenetic embryos in vitro. And the same method of parthenogenesis activation（PA） was adopted within the whole groups. NCSU-23 basic culture medium without addition was taken as control group, while the experiment group were divided into three groups and supplemented with three kinds of Chinese medicine simplex components respectively in vary concentrations at low, moderate and high level（ Lig1, Lig2, Lig3 ; BR1, BR2, BR3 ; Ica1, Ica2, Ica3）, so that the optimal concentration of each experiment group can be screen out. The results showed： ①The PA embryos on 48 h and day 7th, the cleavage and blastocyst rate of Ligl group were significantly higher than Lig2 group and the control group （P 〈 0. 05）, extremely higher than Lig3 group （P〈 0.01）. ② The cleavage rate of BR2 group was significantly higher than the control group and BR3 group, there was no statistical difference between BR1 and BR2 group（P〉0.05）. The blastocyst rate of BR2 group was remarkably higher than any other groups（P〈0.05）, the blastocyst rates among the rest of groups were found no statistically different（P〉0.05）. ③The PA embryos on 48 h and day 7th, the cleavage and blastocyst rate of Ica2 group was significantly higher than any other groups（P〈 0.05） ,and there was no statistical difference between the rest of groups（P〉0.05）. Above all, NCSU-23 basic culture medium supplemented with three kinds of Chinese medicine simplex components could notably improve the development of porcine erabryos in vitro.

关 键 词：猪 中药单体成分 孤雌激活 体外发育 

分 类 号：S814.8[农业科学—畜牧学]