慢性瘤胃酸中毒状态下奶山羊瘤胃细菌内几种相关细菌数量变化的研究  

Quantitative Changes of Some Related Bacteria Species in the Rumen of Dairy Goatssuffered from Subacute Rumen Acidosis

作　　者：刘烨彤[1] 刘大程[1] 卢德勋[2] 胡红莲[2] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018 [2]内蒙古农牧业科学院,呼和浩特010030

出　　处：《中国畜牧兽医》2009年第3期123-126,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(30560105)

摘　　要：研究慢性瘤胃酸中毒(subacute rumen acidosis,SARA)状态下,相关瘤胃微生物数量的变化。将6只体重相近,体况良好,并安装有永久性瘤胃瘘管的泌乳期关中奶山羊作为试验对象,以逐渐增加精料的方式诱导动物发生SARA。试验分4期进行,4期日粮的精粗比分别为5∶5、6∶4、7∶3和8∶2。分别采用16SrRNA探针杂交法和传统培养法对SARA状态下瘤胃内微生物数量变化进行测定。随着日粮精料比例的增加,牛链球菌、乳酸杆菌、反刍兽新月单胞菌、埃氏巨型球菌和淀粉分解菌的数量出现不同程度的增加,而3种主要纤维分解菌的数量有所下降,其中淀粉分解菌的数量极显著增加(P<0.01)。在SARA状态下瘤胃细菌总数、乳酸产生菌和乳酸利用菌数量均有所增长,同时3种主要纤维分解菌的数量明显下降。The quantitative changes of some related bacteria in the rumen of dairy goats suffered from subacute rumen acidosis （SARA） were determined. Six Guanzhong lactating goats with similar weights and fixed with ruminal cannula were used. They were fed concentrates daily by gradually increasing feeding regime. The trial was divided into four stages and in first stage dietary concentrate-to-forage ratio is 5 ： 5, in second stage 6 ： 4, in third stage 7 ： 3,and in the fourth stage 8 ： 2. The amount of ruminal bacteria of the experimental animals were determined by the method of 16S rRNA probes and traditional cultivation method. With the increase of the dietary concentrate to forage ratio, the quantities of Strepococcus boris, Lactobacillus, Selenornonas ruminantium, Megasphera elsdenii and Amylolytic bacteria were increased in varying degrees, while amounts of the three kinds Cellulolytie bacterias were decreased and the numbers of amylolytic bacteria increased signifieantly（P〈0.01）. Under the condition suffered from SARA, the amounts of rumen bacteria, Lactate-production bacteria and Lactate-utilizing bacteria were all increased, and quantities of the; three main kinds Cellulolytic bacterias were decreased significantly.

关 键 词：慢性瘤胃酸中毒 瘤胃微生物 16S rRNA探针 

分 类 号：Q934[生物学—微生物学]