人肝线粒体天冬氨酸氨基转移酶提纯及比活性研究  

PURIFICATION AND SPECIFIC ACTIVITY OF MITOCHONDRIAL ASPARTATEAMINO TRANSFERASE FROM HUMAN LIVER

作　　者：李绍冰[1] 魏素珍[2] 方丁 

机构地区：[1]河北省人民医院老年病科 [2]河北医科大学基础所

出　　处：《河北医科大学学报》1998年第3期149-152,共4页Journal of Hebei Medical University

摘　　要：目的：建立人肝线粒体型天冬氨酸氨基转移酶（ｍＡＳＴ）提纯方法，制备兔抗人ｍＡＳＴ抗血清，为免疫法测定人血清ｍＡＳＴ作准备。方法：采用ＤＥＡＥ纤维素柱层析及亲和层析从人肝中提纯ｍＡＳＴ。应用淋巴结注射法制备兔抗人ｍＡＳＴ抗血清。结果：ｍＡＳＴ比活为３０００００Ｕ／ｍｇ蛋白，达到电泳纯和免疫纯，抗血清效价为１∶１６。结论：ＤＥＡＥ纤维素柱层析及亲和层析能够制备高纯度人ｍＡＳＴ，制备的兔抗人ｍＡＳＴ抗血清效价较高，为建立免疫方法测定ｍＡＳＴ创造了条件。Objective:To establish the methods for the purification of mAST and prepare rabbit antiserum against human mAST so as to set the basis for the measurement of human serum mAST with immunomethod.Methods:The mAST was purified by DEAEcellulose column chromatography and affinity chromatography from human liver.Results:The specific activity of purified mAST is 300 000U/mg protein.The preparation of mAST was identified to be homogeneous by SDSPAGE,PAGE and immunomethod.The titer of the rabbit antihuman mAST serum was 1∶16.Conclusion:Human liver mAST can be highly purified by DEAEcellulose column chromatography and affinity chromatography.The purified mAST and antiserum are of great value in the measurement of human serum mAST by immunomethod.

关 键 词：肝线粒体 天冬氨酸 氨基转移酶 提纯m-AST 

分 类 号：Q555.03[生物学—生物化学]