哮喘患者及豚鼠模型血浆肺组织内皮素1测定及亚硝基左旋精胺酸甲酯的影响  

Detection of ET 1, TNF α, oxidative radicals in sera of asthmatics and in sera and lung tissue of asthmatic guinea pigs

作　　者：杜捷夫[1] 崔德健 郭英江 吕国平 陆连荣 王亚平 

机构地区：[1]解放军第三○四医院呼吸科

出　　处：《中华结核和呼吸杂志》1998年第5期293-296,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的研究哮喘发作患者血浆和豚鼠哮喘模型血浆、肺组织内皮素１（ＥＴ１）、肿瘤坏死因子α（ＴＮＦα）、丙二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）的变化及一氧化氮（ＮＯ）合成酶抑制剂亚硝基左旋精胺酸甲酯（ＬＮＡＭＥ）对其影响。方法选择患者及建立豚鼠哮喘模型，应用放射免疫法测定ＥＴ１，酶联法测定ＴＮＦα，硫代巴比妥酸法及羟胺氧化法测ＭＤＡ和ＳＯＤ。结果哮喘患者血浆ＥＴ１、ＴＮＦα及ＭＤＡ分别为３３±１５（×１０９ｇ／Ｌ），３．１８±０．６５（×１０９ｇ／Ｌ），８．２±１．５（×１０６ｍｏｌ／Ｌ）显著高于对照组１４±４（×１０９ｇ／Ｌ），２．３４±０．０８（×１０９ｇ／Ｌ），４．９±１．３（×１０６ｍｏｌ／Ｌ，Ｐ＜０．０１）；而哮喘患者血浆ＳＯＤ活力为１３５±２６（×１０－３ＮＵ／Ｌ），显著低于对照组１６１±１９（×１０－３ＮＵ／Ｌ，Ｐ＜０．０１）。豚鼠模型血浆、肺组织ＥＴ１含量为１２７±８（×１０９ｇ／Ｌ），２４３±５８（×１０８ｇ／ｇ）；ＴＮＦα含量为３．５０±０．１３（×１０９ｇ／Ｌ），３２．４±３．５（×１０８ｇ／ｇ）；ＭＤＡ含量为２１３±４３（×１０６ｍｏｌ／Ｌ?Objective To investigate the role of the ET 1、TNF α and oxidative radicals of MDA and SOD in the pathogenesis of asthma and the effect of the nitric oxide synthase inhibitor, L NAME to above factors. Method ET 1 was detected by the radio imunoassay and TNF α was detected by ELISA, SOD and MDA were detected by using thiobarbituric acid method. Result The contents of ET 1, TNF( andMDA in the serum of the asthmatic patients and in the serum and lung tissue of asthma models of guinea pigs were significantly increased, in the contrast ,the content of SOD was decreased compared with those in the controls( P <0.01). All the ehanges in the serum and in the lung tissues of the asthma models of guinea pigs returned to the levels of the controls by dexamethasone inhalation or L NAME by intraperitoneal injection. Conclusion ET 1, TNFα and the oxidative radicals and NO may play important roles in the pathogenesis of asthma, L NAME, which is similar to the dexathamethsone, may either decrease the content of NO or decrease the content of ET 1, TNFα and oxidative radicals in asthma.

关 键 词：哮喘 内皮素 一氧化氮 肿瘤坏死因子 

分 类 号：R562.25[医药卫生—呼吸系统]