缺氧性腺泡内肺动脉构形重组的形态定量研究  

Morphometric investigation on hypoxic structural remodeling of intraacinar pulmonary arteries

作　　者：席思川[1,2] 阮英茆[1,2] 刘玉和[1,2] 张连庄[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院病理科 [2]吉林医学院

出　　处：《中华结核和呼吸杂志》1998年第5期303-305,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家"八五"攻关基金

摘　　要：目的观察中药黄芪对缺氧性肺动脉高压及肺腺泡内动脉结构改建的抑制作用。方法６０只大白鼠随机分成缺氧组、缺氧＋黄芪组、正常对照组（各组２０只）。缺氧组、缺氧＋黄芪组大鼠在常压缺氧（１０％Ｏ２１０小时／天）下喂养，在实验第１５天、３０天每组分别取１０只进行右心室收缩压、右心室肥大指数测定后处死，再对肺腺泡内动脉进行光镜、电镜观察及形态学定量分析。结果在实验第３０天后，右心室收缩压，在缺氧组高于缺氧＋黄芪组１８倍（Ｐ＜０．０５），右心室肥大指数，在缺氧组高于缺氧＋黄芪组１３倍（Ｐ＜０．０５），腺泡内动脉中层厚度高于缺氧＋黄芪组２３倍（Ｐ＜０．０５）；肺腺泡内动脉外膜单位面积的纤维母细胞数（密度）在缺氧组是１３１±０３（Ｐ＜０．０５），在缺氧＋黄芪组是７６０±０１９（Ｐ＜０．０５）。结论黄芪抑制了肺腺泡内动脉壁细胞增生及扩张肺动脉。Objective To observe the inhibitory effects of Radix Astragali (RA) on hypoxic structural remodeling of intraacinar pulmonary arteries (IAPA) and pulmonary hypertension (PHT). Method 60 rats were divided into H (hypoxia) group ( n =20 rats) H+RA group ( n =20) and normal control (C) group ( n =20). The rats of H group and H+RA group were fed in hypoxic enviroment under normal atmospheric pressure (10% O 2, 10 hours/day). On the 15th and 30th day of hypoxia, right ventricular systolic pressure (RVSP) and right ventricle hypertrophy index (RVHI) were measured and pulmonary vessel changes were studied under light and electron microscope with morphometric analysis. Result Compared with H+RA group on 30th day, RVSP in H was 1.8 time as high as that of H+RA( P <0.05); RVHI in H was 1.3 time as high as that of H+RA ( P <0.05); the media thickness of IAPA in H was 2.3 time as high as that of H+RA ( P <0.05); the density of adventitial fibroblasts of IAPA was 13.1±0.3 (H) and 7.6±0.2 (H+RA) ( P <0.05). Conclusion Preserving the IAPA wall cells and dilating IAPA by RA may play an important role in inhibiting remodeling of IAPA and PTH.

关 键 词：缺氧症 肺性高血压 肺动脉结构 形态定量研究 

分 类 号：R543.2[医药卫生—心血管疾病]