博莱霉素致大鼠肺泡上皮通透性的改变及糖皮质类固醇对其的影响  

Bleomycin induced changes of lung epithelial permeability in rats and the effects of glucocorticosteroid on it

作　　者：李尔然[1,2] 刘又宁[1,2] 田嘉禾 朱宝华[1,2] 张文娟 于润江[1,2] 

机构地区：[1]中国医科大学第一临床学院呼吸疾病研究所 [2]解放军总医院呼吸科

出　　处：《中华核医学杂志》1998年第2期80-82,共3页Chinese Journal of Nuclear Medicine

摘　　要：目的观察博莱霉素（ＢＭ）致肺间质纤维化大鼠肺泡上皮通透性（ＬＥＰ）的改变及糖皮质类固醇（ＧＣ）对其的影响。方法将５８只成年雄性Ｗｉｓｔａｒ大鼠分为对照组及疾病组（包括７天、１４天非治疗组及ＧＣ治疗组）。气管内一次性注入ＢＭ，制作肺间质纤维化模型，雾化吸入９９ｍＴｃＤＴＰＡ气溶胶测定ＬＥＰ。结果ＧＣ治疗组第７天时，ＬＥＰ较非治疗组降低，支气管肺泡灌洗液中白蛋白及碱性磷酸酶较非治疗组明显下降，但仍高于对照组；ＬＥＰ与支气管肺泡灌洗液中白蛋白及碱性磷酸酶呈正相关（ｒ＝０７８和０７６，Ｐ均＝００１）。结论ＧＣ可降低ＢＭ致肺间质纤维化早期的ＬＥＰ；ＬＥＰ愈高，肺泡上皮的损伤愈重，ＬＥＰ可作为检测肺泡上皮损伤的敏感。Purpose To observe changes of lung epithelial permeability (LEP) and effects of glucocorticosteroid (GC) on it in bleomycin(BM) induced pulmonary fibrosis rats Methods Fifty eight adult male Wistar rats were divided into control group and experimental group (subdivided into 7day, 14day non treated groups and 7day, 14day GC treated groups) The rat pulmonary fibrosis was induced by a single intratracheal instillation of BM LEP was measured by inhalation of 99m Tc DTPA aerosol Results Seven days after injection of GC, alkaline phosphatase (AKP) in broncho alveolar lavage fluid (BALF) and LEP of GC treated group were lower than those of non treated group,but in comparison with those of control group, they are still higher; LEP has a positive correlation with AKP in BALF Conclusions This study suggests: ①at early stage, GC can reduce LEP of BM induced pulmonary fibrosis; ②the severer the damage of lung epithelium is, the higher LEP increases; and LEP can be used as a sensitive and noninvasive index for detecting lung epithelial damage

关 键 词：博来霉素 肺纤维化 肺泡上皮通透性 抗癌药 

分 类 号：R979.14[医药卫生—药品]