兔足冻伤后抗氧化系统的变化  

CHANGES IN ANTIOXIDATIVE SYSTEM IN RABBITS AFTER FROSTBITE

作　　者：杨成君[1] 吕薇[1] 尹旭辉[1] 姜在福[1] 

机构地区：[1]沈阳军区后勤部军事医学研究所

出　　处：《解放军预防医学杂志》1998年第2期93-96,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

摘　　要：为观察兔足冻伤后抗氧化系统的变化，对兔足Ⅰ～Ⅳ度冻伤前及冻伤后１２、２４、４８、７２ｈ时血中超氧化物岐化酶（ＳＯＤ）、过氧化氢酶（ＣＡＴ）、谷胱甘肽过氧化氢酶（ＧＳＨ－Ｐｘ）的活力和丙二醛（ＭＤＡ）的含量进行测定。结果表明：（１）各度冻伤组冻后１２ｈ时，ＳＯＤ、ＣＡＴ、ＧＳＨ－Ｐｘ活性和ＭＤＡ含量均较冻前对照值明显升高，Ｐ＜０．０１。（２）轻度（Ⅰ、Ⅱ度）冻伤各指标变化小于重度（Ⅲ、Ⅳ度），且在冻后２４ｈ恢复到冻前水平。（３）重度冻伤各组ＳＯＤ活性在冻后２４ｈ达到高峰，与冻前比Ｐ＜０．０１，４８ｈ则下降到低于冻前，Ｐ＜０．０５。ＣＡＴ、ＧＳＨ－Ｐｘ活性在冻后２４ｈ虽稍有下降，仍显著高于冻前，Ｐ＜０．０１。Ⅳ度冻伤组于７２ｈ时显著低于冻前，Ｐ＜０．０５。重度冻伤后ＭＤＡ一直持续高于冻前，Ｐ＜０．０１。I through Ⅳ degree of freezing injury were each induced on hind feet of 4 groups of rabbits respectively. The activities of SOD, CAT, and GSH-P X and the content of MDA in blood were determined before(control) and at 12, 24, 48, and 72 h after frostbite to observe the effects on antioxidative system.(1) It was found that 12 h after frostbite, the above mentioned parameters were increased well above the controls,P<0.01.(2) For the mild and moderate groups(I and II degree of cold injury) the changes were less pronounced as in the severely injured groups (III and Ⅳ degree). Recovery took place at 24 h.(3) While in severely injured groups, the activity of SOD reached its peak at 24 h and since 48 h it declined to the level lower than that of control,P<0.05. The activities of CAT and GSH-P X at 24 h showed somewhat decrease but still remained high enough than controls,P<0.01. In Ⅳ degree group the activities of these two enzymes were lower than the control values at 72 h (P<0.05)and plasma content of MDA continuosely maintained at significantly high level after frostbite(P<0.01 vs. control). These results suggested the magnitude and time course of the changes of antioxidative systems in rabbit after frostbite were closely related to the degree of injury.

关 键 词：冻伤 足冻伤 抗氧化系统 SOD CAT GSH-PX 

分 类 号：R645[医药卫生—外科学]