桑树斑状叶色突变体Cty-Sm差异蛋白质组的分析  

Analysis of Differential Proteome of a Leaf-variegated Mutant Cty-Sm in Mulberry(Morus L.)

作　　者：方向明[1] 贾俊丽[1] 谈建中[1,2] 阚雪芹[1] 刘艳艳[1] 池旭娟[1] 平野久[3] 

机构地区：[1]苏州大学建筑与城市环境学院,江苏苏州215123 [2]苏州大学蚕桑研究所,江苏苏州215123 [3]横滨市立大学

出　　处：《蚕业科学》2009年第1期1-5,共5页ACTA SERICOLOGICA SINICA

基　　金：公益性行业(农业)科研项目(编号nyhyzx07-020-03);日本学术振兴会资助项目(编号P04221)

摘　　要：为探讨桑树斑状叶色突变体Cty-Sm叶色变异的分子机制,以该突变体与正常桑的叶片为材料,采用一维电泳-液相色谱-质谱技术对二者的蛋白质组差异表达进行了分析与鉴定。结果表明,与正常绿色叶相比,在桑树斑叶突变体的叶片黄化部位,核酮糖1,5-二磷酸羧化酶/加氧酶(rubisco)大小亚基、光系统Ⅱ捕光色素蛋白、质体蓝素、三磷酸腺苷(ATP)合酶等蛋白质组分的表达量显著减少,而磷酸甘油酸激酶、Rubisco活化酶、核苷二磷酸激酶、Cu-Zn超氧化物歧化酶(SOD)、热激蛋白、18 kD蛋白A及甘露糖结合凝集素等蛋白组分显示上调表达。依据实验结果,探讨了桑树斑叶突变体Cty-Sm的突变类型与蛋白质组差异表达的生理学意义。To explore the molecular mechanism of leaf color variation in mulberry leaf-variegated mutant Cty- Sm, differential expression of the leaf proteome was analyzed and identified through 1 DE-LC-MS technology, using leaves of the mutant and normal mulberry as materials. Comparing with the normal green leaves, the expression levels of several proteins in the etiolated parts reduced significantly, including Rubisco large and small subunits, light-harvesting complex II , plastocyanin and ATP synthase, while expression of other pro- teins was up-regulated, including phosphoglycerate kinase, Rubisco activase, nucleoside diphosphate kinase, Cu-Zn SOD, 17.3 kD class I heat shock protein, 18 kD winter accumulating protein A and mannose- binding lectin. Based on the above results, physiological significance of the differential proteome expression and the mutation type of mulberry leaf-variegated mutant Cty-Sm were discussed.

关 键 词：桑树 斑叶突变体 差异蛋白质组 质谱分析 一维电泳-液相色谱-质谱 

分 类 号：S888[农业科学—特种经济动物饲养]