rhEPO和rhG-CSF对小鼠CD90^+细胞的动员作用  

Effects of rhEPO and rhG-CSF on Mobilization of CD90^+ Cells in Mice

作　　者：郑芳[1,2] 陈龙[3] 程范军[2] 刘岐焕[2] 张有顺[3] 邹灿[3] 

机构地区：[1]武汉大学人民医院血液科,湖北武汉430060 [2]郧阳医学院附属东风医院血液科,湖北十堰442008 [3]郧阳医学院附属东风医院实验中心,湖北十堰442008

出　　处：《郧阳医学院学报》2009年第1期1-4,共4页Journal of Yunyang Medical College

基　　金：湖北省自然科学基金(200800156);湖北省科技攻关项目(2007AA301C14-1);湖北省杰出青年科技人才基金项目(QJX2008-40);郧阳医学院优秀中青年科技创新团队项目(2008CXX02)

摘　　要：目的:探讨重组人促红细胞生成素(rhEPO)和重组人粒细胞集落刺激因子(rhG-CSF)对小鼠外周血CD90+细胞的动员作用。方法:30只雄性昆明小鼠随机分为对照组、rhG-CSF组和rhEPO组。rhG-CSF 40μg.kg-1.d-1、rhEPO 250 IU.kg-1.d-1连续皮下注射5 d;对照组连续皮下注射生理盐水。每组均于最后一次注射后6 h收集外周血,计数有核细胞(ANC)和单个核细胞(MNC),流式细胞仪检测外周血ANC中CD90+、CD34+CD90+和CD90+CD34-细胞的比例。结果:应用rhG-CSF和rhEPO动员后,小鼠外周血中ANC和MNC的数量均较对照组增加(P<0.05),但rhG-CSF和rhEPO两组之间无差异(P>0.05)。流式细胞术检测显示rhEPO组外周血ANC中CD90+细胞比例为(56.49±9.06)%,较rhG-CSF组明显增加(P<0.05);而rhG-CSF组和对照组CD90+细胞占ANC的比例无明显差异(P>0.05)。rhEPO组的CD90+CD34-细胞比例增至(53.5±8.9)%,占CD90+细胞中绝大多数。结论:rhEPO和rhG-CSF均有较强的造血干细胞动员能力,但rhEPO对CD90+细胞的动员作用强于rhG-CSF,提示rhEPO对早期造血干细胞可能具有更强的动员作用。Objective To investigate effects of recombinant human erythropoietin（rhEPO） and recombinant human granulocyte colony stimulating factor（rhG-CSF） on mobilization of CD90^＋ cells in mice, and compare their mobilization efficiency. Methods Thirty male Kunming mice were randomly divided into control, rhG-CSF and rhEPO groups. The mice were subcutaneously injected with rhG-CSF in a dose of 40 μg·kg^-1·d^-1 ,rhEPO in a dose of 250 IU·kg^-1·d^-1 or saline for 5 days,respectively. The peripheral blood were collected at six hours afer the final injection, the number of absolute nuclear cells （ANC） and mononuclear cells （MNC） were counted, the proportion of CD90^＋ , CD34^＋ CD90^＋ and CD90^＋ CD34^- cells in ANC was detected by flow cytometry. Results Compared with control group,the number of ANC and MNC in peripheral blood were increased in both rhG-CSF group and rhEPO group （ P 〈 0.05 ） , but there was no significant difference between rhG-CSF and rhEPO group （ P 〉 0.05 ）. The proportion of CD90^＋ cells in rhEPO group was （ 56.49±9.06 ） %, and significantly increased than that of rhG-CSF group （P 〈 0.05 ） ;while the proportion of CD90^＋ cells in ANC had no significant difference between rhG-CSF group and control group （ P 〈 0.05 ）. The proportion of CD90^＋ CD34^- in rhEPO group was increased to （53.5±8.9） % and was accoutable for the majority of CD90^＋ cells. Conclusion Both rhG-CSF and rhE- PO have a strong ability of hematopoietic stem cells mobilization, the mobilization effect of rhEPO was more greater than rhG-CSF in CD90^＋ ceils mobilization, which suggests that rhEPO could mobilize primitive hematopoietic stem ceils more effectively.

关 键 词：促红细胞生成素 粒细胞集落刺激因子 动员 外周血 造血干细胞 

分 类 号：R392.4[医药卫生—免疫学]