肠胃清对人大肠癌细胞HCT8/V皮下移植瘤长春新碱耐药的逆转作用  

Reversal Effect of "Changweiqing Fluid" on Vincristine-risistance in Human Colorectal Cancer Cell Line HCT-8/V Tumor Xenograft Mice

作　　者：许建华[1] 邓皖利[1] 孙珏[1] 张勇[1] 李朝衡[1] 范忠泽[1] 

机构地区：[1]上海中医药大学附属普陀医院肿瘤科,上海200062

出　　处：《上海中医药大学学报》2009年第2期59-63,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672683);上海市教委科研基金资助项目(05CZ16)

摘　　要：目的:观察中药复方肠胃清口服液逆转大肠癌长春新碱耐药的作用,初步探讨其逆转耐药的作用机制。方法:建立人大肠癌耐长春新碱(VCR)细胞株HCT8/V裸鼠皮下移植瘤模型,随机分为正常对照组、VCR组、肠胃清组、肠胃清高剂量+VCR组、肠胃清低剂量+VCR组。治疗结束后计算瘤体抑制率;高效液相色谱法检测瘤内VCR药物浓度;流式细胞技术检测瘤内细胞周期和凋亡改变。结果:肠胃清高低剂量联合VCR的瘤体抑制率为47.47%、69.62%,明显高于单用肠胃清或VCR组;对照组VCR的含量为0.1745μg/g瘤重,而肠胃清高剂量联合VCR组的浓度为12.2236μg/g瘤重,是对照组的70.05倍;与对照组比较,药物干预后,G1期细胞明显增加,S期细胞明显低于对照组,细胞凋亡率明显增加,以肠胃清联合VCR组作用最强。结论:肠胃清逆转大肠癌移植瘤对VCR的耐药与通过增加瘤体内药物浓度、诱导细胞凋亡有关。Objective： To investigate the reversal effect of ＂Changweiqing Fluid＂ （CWQ） on muhidrug resistance in human colorectal cancer cell and its mechanisms. Methods： Establish xenograft model of the vincristine（VCR）-risistant human colorectal cancer cell line HCT-8/V in nude mice. Then the mice were randomly divided into five groups： control group （NS）, CWQ group, VCR group, lower dose CWQ ＋ VCR group and higher dose CWQ ＋ VCR group. After treatment, the inhibition rate of tumor in each group were calculated. Intracellular VCR concentration in HCT-8/V cells was detected by HPLC; the diversify of the cell cycle and apoptosis was detected by FCM. Results： The inhibition rates in lower dose CWQ ＋ VCR group and higher dose CWQ ＋ VCR group were 47.47% , 69.62% respectively, which were significantly higher than that of CWQ or VCR groups. The concentration of VCR in NS group was 0. 174 5 μg/g of tumor weight, and in higher dose CWQ ＋ VCR group the concentration was 12. 223 6 μg/g, as high as 70.05 times of the NS group. Compared with control group, after drug intervention, ceils in G1 phase increased markedly and ceils in S phase decreased significantly, and the rate of apoptosis increased significantly, with the biggest change in CWQ ＋ VCR group. Conclusion： The reversal of VCR resistance in human colorectal cancer by ＂Changweiqing＂ （CWQ） may be associated with its inereasing drug concentration in tumor and inducing cell apoptosis.

关 键 词：肠胃清 大肠癌 多药耐药 长春新碱 皮下移植瘤 

分 类 号：R285.5[医药卫生—中药学] R735[医药卫生—中医学]