肾上腺髓质素对新生大鼠缺氧缺血脑损伤的作用  

Effect of adrenomedullin on brain damage induced by hypoxic ischemic encephalopathy in neonatal rats

作　　者：许春花[1] 金正勇[1] 李秋萍 

机构地区：[1]延边大学附属医院儿科,吉林延吉133000 [2]吉林省延吉市延东路门诊

出　　处：《中国妇幼保健》2009年第10期1409-1410,共2页Maternal and Child Health Care of China

摘　　要：目的:探讨外源性肾上腺髓质素(Adrenomedullin,ADM)对缺氧缺血新生大鼠脑组织中SOD和NO水平的影响及ADM治疗新生大鼠缺氧缺血性脑损伤(HIBD)的作用机制。方法:将7日龄Wistar大鼠100只随机分为正常组、HIBD模型组、生理盐水对照组(NS组)、丹参干预组(丹参组)、肾上腺髓质素干预组(ADM组),制备HIBD模型后注射ADM及丹参注射液。缺氧缺血后72 h处死,取大脑皮质,制作脑组织匀浆,检测脑组织中SOD、NO水平。结果:HIBD组与NS组脑组织中SOD水平低、NO水平高,同正常组比较有显著性差异(P<0.01),ADM、丹参组SOD水平高、NO水平低,同模型组比较有显著性差异(P<0.01)。结论:ADM可通过血脑屏障,能使大脑皮质SOD水平升高,降低NO水平,对HIBD有防治作用。Objective： To explore the effect of exogenous adrenomedullin on the superoxide dismutase （SOD） and nitric oxide （NO） levels in brain tissues of hypoxic -ischemic neonatal rats, explore the treatment mechanism of adrenomedullin on neonatal rats with brain damage （HIBD） . Methods： 100 neonatal Wistar rats aged 7 days were divided into normal group, HIBD group, normal saline control group, salvia mihiorrhiza group and adrenomedullin group, randomly. HIBD models were established, then adrenomedullin and salvia mihiorrhiza were injected intraperitoneally and subcutaneously, the rats were killed after hypoxia and ischemia for 72 hours. SOD and NO levels in brain tissues were detected. Results： SOD levels of brain tissues in HIBD group and normal saline group were lower than that in normal group, and NO levels of brain tissues in HIBD group and normal saline group were higher than that in normal group （P 〈0. 01 ） ; SOD levels in adrenomedullin group and salvia mihiorrhiza group were higher than that in HIBD group, and NO levels in adrenomedullin group and salvia miltiorrhiza group were lower than that in HIBD group （P 〈 0. 01 ） . Conclusion： Adrenomedullin can pass through the blood - cerebral bartier, increase the level of SOD and reduce the level of NO in cerebral cortex, so it plays a role in the prevention of HIBD.

关 键 词：超氧化物歧化酶 一氧化氮 肾上腺髓质素 脑缺氧 脑缺血 

分 类 号：R971.93[医药卫生—药品]