川芎嗪对百草枯中毒大鼠肾组织核因子-кB及iNOS表达的影响  

Effects of ligustrazine on the expression of nuclear factor-kappa B and iNOS in rats with acute kidney injury induced by Paraquat

作　　者：刘勇涛 刘美娟[2] 刘会芳[3] 赵燕燕[3] 马焕云[3] 

机构地区：[1]高阳县医院,河北高阳071500 [2]河北大学附属医院 [3]河北大学医学部

出　　处：《山东医药》2009年第9期22-24,共3页Shandong Medical Journal

基　　金：河北省中医药管理局科研基金资助项目(2008070;2008072);河北省卫生厅科研基金资助项目(08370);河北省科技攻关项目(05276101D-88;42761220);河北大学医学部科学研究基金资助项目(2007Q06)。

摘　　要：目的探讨川芎嗪对急性百草枯中毒大鼠肾损伤时核因子-кB(NF-кB)和诱导型—氧化氮合酶(iN-OS)活性的影响。方法将50只SD大鼠随机分成空白组、阴性对照组、阳性对照组、川芎嗪低剂量组和川芎嗪高剂量组,对肾组织标本进行组织病理学检查,同时测定肾组织NF-кB和iNOS活性。结果与阴性对照组相比,川芎嗪低剂量组肾组织病理显示肾间质充血明显减轻,NF-кB和iNOS也降低(P<0.01);而川芎嗪高剂量组无明显改善。结论NF-кB及iNOS在百草枯所致大鼠肾损伤中起重要作用,川芎嗪能降低肾组织NF-кB及iNOS水平,减轻百草枯中毒大鼠的肾组织损伤。Objective To investigate the effects of ligustrazine on nuclear factor-kappa B （NF-κB） and inducible nitric oxide synthase （iNOS） in rats with kidney injury induced by Paraquat. Methods 50 Sprague- Dawley （SD） rats were randomly divided into five experimental groups ： blank group, negative control group, positive control group, low-dose of lignstrazine group（LDG） and high-dose of ligustrazine group （HDG）. Macroscopicand histopathological examinations were performed. In the mean time, the activities of hepatic tissue homogenate NF-κB and iNOS were measured. Results Compared with that in negative control group, nephredema of low-dose of ligustrazine group were mitigated, and the contents of NF-κB and iNOS were significantly decreased （ P 〈0.01 ）. That of high-dose of ligustrazine group did not changed significantly. Conclusions NF-κB and iNOS could play an important role in kidney injury of poisoned rats. Ligustrazine could ameliorate the kidney injury and decrease the concentrations of NF-κB and iNOS.

关 键 词：川芎嗪 百草枯 肾损伤 核因子-ΚB 一氧化氮合酶 

分 类 号：R163.3[医药卫生—公共卫生与预防医学]