骨髓间质干细胞突变的致瘤肿瘤干细胞分离及培养  

Isolation and culture conditions of cancer stem cells in F6 mutatant line from bone marrow mesenchymal stem cells

作　　者：徐学静[1] 许文荣[1] 钱晖[1] 朱伟[1] 张徐[1] 曹慧玲[1] 陈忠[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《临床检验杂志》2009年第2期118-120,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(30840053);卫生部科研课题(WKJ2005222024);镇江市重点实验室(2006066);江苏省医学领军人才(LJ200614);江苏省自然科学基金(BK2007705);江苏省自然科学基金(BK2007092;BK2008232);镇江市社会发展基金(SH2005064);江苏大学研究创新基金

摘　　要：目的建立合适的骨髓间质干细胞突变的肿瘤细胞系F6(肿瘤干细胞)分离及体外长期培养方法并探讨其生物学特性。方法采用抗CD133-PE抗体经流式细胞仪分选F6肿瘤干细胞(F6 CSCs),研究不同培养体系对F6 CSCs体外生长的影响,探讨最适体外培养条件。结果一次分选获得F6 CSCs纯度达到80%。分选的F6 CSCs对Balb/c小鼠有体内致瘤能力,在含2%胎牛血清的L-DMEM营养液中可以长期稳定的培养,其CD133表面标志的表达维持在68.87%。结论低浓度胎牛血清的培养液能维持F6 CSCs的生长并保留其相应的生物学特性,这为肿瘤干细胞体外培养及研究提供了新的思路和方法。Objective To isolate the cancer stem cells in F6 cell line, optimize the culture conditions of F6 cancer stem cells （CSCs）, and explore whether it can maintain CSCs characteristics in the culture medium. Methods Sorting CD133 ＋ cells from F6 cell line were detected by flow cytometric analysis. The appropriate culture conditions for F6 CSCs were screened from three kinds of medium. Results CD133 ＋ F6 CSCs had higher tumorgenicity than CD133-F6 cells, when transplanted into BALB/c nude mice. CD133 ＋ F6 CSCs cultured in L-DMEM supplemented with 2% FBS may maintain the percentage of CD133 expression up to 68.87%. Conclusions The F6 CSCs proliferate and keep the properties under low serum culture condition, e.g. , L-DMEM supplemented with 2% FBS. These findings might provide a novel approach for cancer stem cells culture.

关 键 词：肿瘤干细胞 间质干细胞 CD133 

分 类 号：R446.11[医药卫生—诊断学]