子宫肌瘤和肌壁组织B7-H3 mRNA表达水平及意义  

Expression level and its significance of B7-H3 mRNA in hysteromyoma and muscle wall

作　　者：蔡惠芬[1] 史进方[1] 顾国浩[1] 张光波[1] 樊一笋[1] 成婧[1] 

机构地区：[1]苏州大学附属一院检验科,江苏苏州215006

出　　处：《临床检验杂志》2009年第2期121-123,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的探讨子宫肌瘤患者肌瘤组织和正常子宫肌壁B7-H3 mRNA的表达水平及其在子宫肌瘤患者发病中的作用。方法利用逆转录多聚酶链反应法和实时荧光定量PCR法检测44例子宫肌瘤患者的肌瘤组织和正常子宫肌壁的B7-H3 mRNA表达水平。结果子宫肌瘤的肌瘤组织表达的B7-H3 mRNA高于正常子宫肌壁。结论子宫肌瘤组织高表达B7-H3 mRNA,B7-H3 mRNA高表达可能与子宫肌瘤发生发展有关。Objective To discuss the expression level of B7-H3 mRNA in leiomyoma and normal uterine muscle wall and the role of B7- H3 in pathogenesis of the patients with hysteromyoma. Methods BT-H3 mRNA expression level in leiomyoma and normal uterine muscle wall of 44 hysteromyoma patients was detected by reverse transcription polymerase chain reaction （RT-PCR） and real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）. Results The expression level of BT-H3 mRNA in leiomyoma was significantly higher than that in uterine muscle wall. Conclusion High expression of B7-H3 mRNA in leiomyoma may be related to the development of hysteromyoma.

关 键 词：子宫肌瘤 B7-H3 实时荧光定量PCR 

分 类 号：Q78[生物学—分子生物学]