紫外分光光度法测定贻贝体内石油烃的方法研究  

Study on the determination of petroleum hydrocarbon in mussels by UV spectrophotometry

作　　者：尚龙生[1] 孙茜[1] 徐恒振[1] 周传光[1] 

机构地区：[1]国家海洋环境监测中心

出　　处：《海洋环境科学》1998年第1期62-65,共4页Marine Environmental Science

摘　　要：选择贻贝为试样，经皂化后用ＣＨ２Ｃｌ２萃取，提取物经氧化铝柱脱色，浓缩后的样品溶于正己烷中，用紫外分光光度计在２２５ｎｍ和２５４ｎｍ波长上分别进行测定。结果表明，用波长２５４ｎｍ测定贻贝中的石油烃要比２２５ｎｍ的测定效果好；对脱色实验做了研究，用加标实验法计算了两波长测定结果的回收率（９８．８％～１１０％），检测限分别是０．６２×１０－６（２２５ｎｍ）和０．０５２×１０－６（２５４ｎｍ）。Mussel was used as a sample and dichloromethane as an extractant. The sample extract was decoloured with an alumina columns. The eluate was dried in the decompression using a rotavapor. The residue containing the aromatics was redissolved in aromatie free hexane. The absorption values were measured respectively at 225 nm and 254 nm by UV spectrophotometer. The results show that it is the best wavelength at 254 nm for the determination of petroleum hydrocarbons in mussels by UV. The recovery rates were 98.8%～110% by the saponification,column separation of the samples. The detection limits were 0.62×10 -6 (225 nm) and 0.052×10 -6 (254 nm).

关 键 词：石油烃 贻贝 紫外分光光度法 生物监测 

分 类 号：X835.02[环境科学与工程—环境工程]