复方茯苓甘草汤对肺动脉高压大鼠肺动脉转化生长因子β_1表达的影响  

Effect of Compound Fuling Gancao Decoction on Transforming Growth Factor β_1 in Pulmonary Artery of Rats with Pulmonary Hypertension

作　　者：王伯章[1] 陈恺[2] 范伟赢[1] 

机构地区：[1]广东医学院附属医院中医科,广东湛江524001 [2]广东医学院附属医院老年病科,广东湛江524001

出　　处：《广州中医药大学学报》2009年第1期50-53,102,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药管理局重点课题资助项目(编号:3060007)

摘　　要：【目的】观察复方茯苓甘草汤对肺动脉高压(PAH)大鼠肺动脉转化生长因子-β1(TGFβ-1)表达的影响,以探讨该方防治肺动脉高压、肺血管重构的作用机理。【方法】选用30只SD雄性大鼠随机分为正常对照组、模型组、复方茯苓甘草汤治疗组(中药组,剂量为5.46.gkg-1.d-1);腹腔内一次性注射野百合碱(剂量为50 mg/kg)造模,采用右心导管法检测大鼠平均肺动脉压(pmPA);计算右心肥厚指数(p右心肥厚);采用病理图像分析系统计量肺小动脉形态学指标,免疫组化法观察肺动脉中TGFβ-1的表达。【结果】模型组大鼠pmPA、p右心肥厚显著升高,肌型动脉及部分肌型动脉比例增多,肌型动脉中膜面积与管腔面积比值(AM/AL)增大,肺动脉TGFβ-1表达明显增多;复方茯苓甘草汤组上述各指标均有显著改善。【结论】复方茯苓甘草汤可有效降低肺动脉压、改善肺血管重构,其机理可能与抑制肺动脉TGFβ-1的表达有关。Objective To observe the effect of Compound Fuling Gancao Decoction（CFGD） on transforming growth factor β1（TGF-β1） in pulmonary artery of rats with pulmonary hypertension（PH）,and to explore its therapeutic mechanism for PH and pulmonary vascular remodeling.Methods Thirty SD male rats were randomized into the normal control group,model group,and CFGD（5.46g·kg-1·d-1） group.PH rats models were induced by intraperitoneal injection of monocrotaline（50mg/kg）.The mean pulmonary artery pressure（MPAP） in rats was detected with the method of right cardiac catheterization,right ventricular hypertrophy index was calculated,histological changes of lung small artery were analyzed with pathological image analysis,and TGF-β1 expression in pulmonary artery was detected by immunohistochemical method.Results MPAP and right ventricular hypertrophy index were enhanced obviously,the number of muscular arteries and partially-muscular arteries was increased,ratio of media area and luminal thickness in the muscular arteries became bigger,and TGF-β1 expression in pulmonary artery of the model group was increased.The above indexes were improved in CFGD group.Conclusion CFGD is effective on decreasing pulmonary pressure and improving pulmonary vascular remodeling,and its mechanism is probably related with the inhibition of TGF-β1 expression in pulmonary artery.

关 键 词：复方茯苓甘草汤/药理学 肺动脉高压/中药疗法 肺血管构型重建/中药疗法 肺组织/病理学 转化生长因子Β 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]