GC×ＧC／TOFMS技术在皮暗斑螟性信息素研究中的应用  

Studies on the Sex Pheromone of Euzophera batangensis by GC×GC/TOFMS Analysis

作　　者：温秀军[1] 孙朝辉[1] KALINOVA Blanka JIROS Pavel 刘满光[3] 

机构地区：[1]华南农业大学林学院,广东广州510642 [2]捷克科学院生物有机化学研究所,捷克布拉格16610 [3]河北省林业科学研究院,河北石家庄050061

出　　处：《华南农业大学学报》2009年第2期53-56,共4页Journal of South China Agricultural University

基　　金：中捷政府间科技合作项目(38-14);华南农业大学校长基金项目(4400-208012)

摘　　要：通过收集皮暗斑螟Euzophera batangensiz的处女雌蛾和雄蛾,提取雌蛾性腺分泌物,在室内通过气相色谱与触角电位测定相结合的方法,测定皮暗斑螟雄蛾对来自雌蛾性腺的不同化合物的反应,并应用全二维气相色谱飞行时间质谱分析技术(GC×GC/TOFMS),弄清了皮暗斑螟性信息素组分为(Z9,E12)-十四碳-9,12-二烯-1-醇和(Z9)-十四碳-9-烯-1-醇.研究过程发现,GC×GC/TOFMS与传统气相色谱质谱(GC-MS)分析相比,灵敏度和精度更高,可以达到0.01 ng/μL,在昆虫信息素的研究中具有更大的优势.Jujuba moth, Euzophera batangensis Caradja, is an important insect pest in jujube orchards. It may cause severe damage in jujube orchards and can attack many other plants, such as loquat （Eriobotrya japonica Lindl. ）, Horsetail Tree （Casuarina equisetifolia L. ） and etc. The conventional gas chromatography with eleetroantennographic detection （GC-EAD） and two-dimensional （ GC x GC） gas chromatography using a time-of-flight mass spectrometric detector （TOFMS）, were combined to analyze the female sex pheromone gland extract of the borer. The GC-EAD analysis produced two EAD responses in GC areas where no compounds were detected by FID detection. The GC x GC/TOFMS analysis in this area indicated the presence of several chemicals, including （Z9 ,El2）-tetradeca-9,12-dien-l-ol and （Z9）-tetradec-9-en-l-ol, pheromone components closely related to Euzophera species. The spectral characteristics, retention behavior and the ability to elicit GC-EAD responses implied that both identified unsaturated alcohols were candidates for sex pheromone components of E. batangensis. The GC × GC/TOFMS facilitated the analysis of complex matrices in a high level and would be a great potential tool for analysis of insect pheromones.

关 键 词：皮暗斑螟 性信息素 GC×GC/TOFMS 

分 类 号：S767.33[农业科学—森林保护学]