公猪精液PRRSV变异株检测及NSP2、ORF5基因序列分析  

Detection of PRRSV Variant Strain in Boar Semen and Sequence Analysis of NSP2 and ORF5 Genes

作　　者：王连想[1,2] 孙彦伟 马静云[2] 张冠群[2] 谢青梅[2] 陈锋[2] 毕英佐[2] 于康震[3] 

机构地区：[1]广东省动物防疫监督总所,广东广州510230 [2]华南农业大学动物科学学院,广东广州510642 [3]中国兽医药品监察所,北京100081

出　　处：《华南农业大学学报》2009年第2期109-111,共3页Journal of South China Agricultural University

基　　金：广东省科技计划项目(2005A20902001);广东省农业厅2007年动植物防疫检疫研究专项

摘　　要：用荧光RT-PCR方法从某猪场精液中检测到猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)变异株(NSP2 1 594～1 680 bp缺失)核酸阳性.提取1份阳性样品病毒核酸测定和分析其NSP2和ORF5全基因序列,结果表明NSP2基因由950个氨基酸组成,与CH-1a、VR-2332等经典PRRSV相比481位缺失1个氨基酸、532～560位连续缺失29个氨基酸,与JXA1、HUN4等毒株具有相同的缺失特性;ORF5基因第13、151位为具有强毒特性的精氨酸(R),存在4个潜在的糖基化位点,分别位于30～32、35～37、44～46和51～53位氨基酸.遗传进化分析表明,测定序列与JX-A1、HUN4等毒株关系最近,与CH-1a、NVSL等同属一个大分支,而与BJ-4、P129、RespPRRS MLV、VR-2332等处于不同分支.研究证实公猪精液可携带PRRSV变异株,因此猪场(群)在人工授精和引进精液时必须强化检疫和生物安全措施.Porcine reproductive and respiratory syndrome virus （PRRSV） variant strains （NSP2 1 594 - 1 680 deletion） were identified by real-time RT-PCR from boar semen of a pig farm. eDNA of NSP2 and ORF5 genes of one strain named GDTS were cloned and sequenced. The results showed that NSP2 gene was composed of 950 amino acids, which had 1 amino acid deletion at the positions of 481 and 29 amino acids deletion at the positions of 532-560 compared with that of CH-la and VR-2332, which had the same deletion compared with that of JXA1 and HUN4. Arginines at the positions of 13 and 151 of ORF5 gene were found and indicated high pathogenicity of the virus. Four glycosylation sites, 30 - 32 、35 - 37、44 -46 and 51 -53 amino acids were found in ORF5 gene. These results proved PRRSV variant （NSP2 1 594 - 1 680 deletion） could be transmitted by boar semen and therefore more control strategies should be taken in artificial insemination.

关 键 词：精液 猪繁殖呼吸综合征病毒 变异株 NSP2基因 ORF5基因 

分 类 号：S852.65[农业科学—基础兽医学]