IL-9和IL-5在支气管哮喘中的表达及意义  

Expression and Significance of IL-9 and IL-5 in Asthma

作　　者：冷弘 马超美[2] 胡军[3] 

机构地区：[1]洛阳市卫生学校,河南洛阳471001 [2]洛阳市妇女儿童医疗保健中心,河南洛阳471000 [3]郑州大学基础医学院,河南郑州450001

出　　处：《河南科技大学学报（医学版）》2009年第1期4-6,共3页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的研究白细胞介素-9(IL-9)和白细胞介素-5(IL-5)在支气管哮喘气道炎症中的作用。方法收集支气管哮喘患者痰液40例,正常对照组20例。用夹心酶联免疫吸附法测定诱导痰上清液IL-9、IL-5及IL-8浓度。结果哮喘发作期组IL-9、嗜酸性粒细胞(EOS)、IL-5明显高于哮喘缓解期组(P<0.01);哮喘发作期组和缓解期组的IL-9、IL-5、EOS明显高于正常对照组(P<0.01)。哮喘发作期组IL-9浓度分别与EOS和IL-5浓度呈正相关(r=0.39,P=0.025;r=0.542,P=0.001),哮喘发作期组IL-8浓度与中性粒细胞百分比呈正相关(r=0.881, P<0.001);在哮喘发作期的中、重度患者中,IL-9与IIL-8水平呈正相关(r=0.528,P=0.002)。结论IL-9与哮喘气道内的EOS、IL-5密切相关,在哮喘发作期尤为明显,表明IL-9和IL-5参与哮喘患者气道炎症过程。Objective To investigate the role of interleukin-9 （,IL-9） and interleukin-5 （IL-5） in the pathogenesis of airway inflammation in asthma. Methods Induced sputum was obtained from 40 asthmatics. 20 healthy individuals were enrolled as controls. IL-9, IL-5 and IL-8 in sputum supernatants were assayed by sandwich enzyme-linked immunosorbent assay（ELISA）. Results The counts of EOS,the levels of IL-9,IL-5 in induced sputum of the asthmatics were significantly higher than those in the controls（P 〈 0.01 ）. Furthermore, these parameters were higher in the asthmatics of acute exacerbation than those of stable phase （P 〈 0. 01 ）. In the acute exacerbated asthmatics,a positive correlation was found between the level of IL-9 and the percentage of eosinophile （ r = 0.39, P = 0. 025 ） , the level of IL-5 and IL-9 （ r = 0. 542, P = 0. 001 ） ,the level of IL-8 and the percentage of neutrophil （r =0. 881 ,P 〈0. 001 ） ,and the level of IL-9 and IL-8 （ r = 0. 528, P = 0. 002）. Conclusion IL-9 is increased in the induced sputum from asthmatics, especially in exacerbation with asthma, suggesting that IL-9 and IL-5 may participate in the modulation of asthmatic airway inflammation.

关 键 词：支气管哮喘 白细胞介素-9 白细胞介素-5 气道炎症 

分 类 号：R562.25[医药卫生—呼吸系统]