血管生成因子2在胶质瘤模型中的表达与shRNAs靶向治疗  

The expression of angiopoietin- 2 in glioma model and treatment with shRNAs

作　　者：刘振林[1] 刘晓智[2] 张文彬[2] 张赛[2] 

机构地区：[1]天津医科大学神经外科,300162 [2]武警医学院附属医院神经外科

出　　处：《中华神经外科杂志》2009年第3期209-212,共4页Chinese Journal of Neurosurgery

基　　金：天津市科委科技攻关项目（06YFSZSF01200）

摘　　要：目的检验血管生成因子2（angiopoietin-2，Ang-2）在胶质瘤模型中的表达，应用RNA干扰技术靶向Ang-2，观察胶质瘤治疗效果。方法建立荷瘤小鼠颅内U87胶质瘤模型，检测Ang-2和PCNA的表达；转染shRNAs表达质粒进入U87细胞，比较受试动物生存期，MRI观察肿瘤体积；脑组织行HE染色及免疫组化检测。结果颅内模型中Ang-2在胶质瘤边缘的表达强于肿瘤内部（P〈0．05），围绕于新生血管周围；靶向Ang-2的shRNAs基因治疗可延长受试动物生存期，降低新生血管数量，但PCNA表达无统计学意义（P〉0．05）。结论Ang-2主要表达于胶质瘤侵袭边缘，与肿瘤新生血管形成密切相关；靶向Ang-2的shRNAs技术可有效降低胶质瘤的侵袭能力。Objective To study the expression of angiopoietin- 2 in glioma model and observe treatment effect on glioma model in nude mice with small hairpin RNA targeting Ang- 2. Method The U87 glioma model in nude mice was used to explore the expression of angiopoietin- 2 and PCNA. After shRNAs plasmid was transfected into U87 glioma ceils by liposome, the treatment model was established as mentioned above to detect Ang- 2 and PCNA expression, then the mean survival of mice was observed, and the tumor volume was detected by MRI. Results Glioma cells invaded into normal brain tissue extensively in established glioma model, and Ang- 2 expression was stronger in borderline region of the glioma model than centre, mainly gathered new form vessels. Treatment with small hairpin RNA targeting Ang- 2 can prolong the survival of mice, and inhibit new vessel form. However, the expression of PCNA was no significantly different. Conclusions The expression of Ang- 2 was mainly gathered in the borderline of the glioma model, and closely related with new vessel form. Small hairpin RNA targeting Ang- 2 can decrease remarkably invasive ability of glioma.

关 键 词：血管生成因子2 RNA干扰 神经胶质瘤 侵袭性 

分 类 号：R686[医药卫生—骨科学]