急性百草枯中毒大鼠肺组织炎症因子表达的变化及意义  

Expression of inflammatory factors in lung tissue of acute paraquat poisoned rats

作　　者：贺晓艳[1] 孙琦[1] 李忠旺[1] 卢中秋[1] 洪广亮[1] 梁欢[1] 邱俏檬[1] 胡国新[2] 

机构地区：[1]温州医学院附属第一医院急诊科,325000 [2]温州医学院附属第一医院药理教研室,325000

出　　处：《中华劳动卫生职业病杂志》2009年第3期149-152,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：浙江省医学扶植重点建设学科计划（07-F04）;浙江省教育厅科研计划重点项目（Z200803373）

摘　　要：目的观察急性百草枯（PQ）中毒大鼠肺组织中炎症因子表达的变化，探讨早期及晚期炎症因子在急性PQ中毒肺损伤中的作用。方法50只雄性SD大鼠随机分为2组：正常对照组（10只，生理盐水腹腔注射），PQ染毒组（40只，腹腔注射体积分数为1％的PQ溶液，20mg／kg）。PQ组于染毒后6h及1、3、7d处死，正常对照组于处理后1d处死，检测肺组织中肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）、高迁移率族蛋白-1（HMGB-1）mRNA的表达，并观察大鼠的中毒表现及肺组织的病理改变。结果PQ染毒组大鼠肺组织病理表现为早期充血水肿明显，大量炎性细胞浸润。与正常对照组相比，PQ染毒组大鼠肺组织中TNF-α、IL-10、HMGB-1 mRNA的表达在各时间点均有不同程度升高。PO染毒组6h和1d时TNF-α mRNA表达分别为0．740±0．100和0．584±0．049，1、3d时IL-10mRNA表达分别为0．551±0．016和0．524±0．010，6h及1、3d时HMGB-1 mRNA表达分别为0．695±0．060、0．871±0．154和0．819±0．188，与对照组比较，均明显升高，差异均有统计学意义（P〈0．05或P〈0．01）。结论大鼠急性PQ中毒后肺组织中TNF-α、IL-10及HMGB-1 mRNA的表达明显上调，早期及晚期炎症因子均参与PQ急性中毒肺损伤的致病过程。Objective To investigate the expression of inflammatory factors in lung tissue of acute paraquat （PQ） poisoned rats. Methods Fifty male SD rats were randomizedly divided into two groups： the normal control group （NC group ,n=10） and the PQ group（n=40）. The 1 ml saline was administered once in normal control group. The PQ group was administered with 20 mg/kg 1%PQ by intraperitoneal injection to establish the model of PQ induced lung injury. At six hours, at the first, the third and the seventh day the PQ group were sacrificed, While at the first day the normal control group was sacrificed. The level of tumor necrosis factor of（TNF-α）mRNA, interleukin 10（IL-10） mRNA, high mobility group box 1 （HMGB-1）mRNA in lung of rats were detected. Meanwhile, pathological changes of the lung were examined under optical microscope. Results Compared with that in normal control group, TNF-α mRNA expression in lung tissue of PQ group reached the peak at the six hour and decreased slowly at the first day[（0.740±0.100） and（0.584±0.049） re- spectively]. At the six hour and the first day in PQ group it was significantly higher than that in normal control group （ P〈0.05 or P〈0.01）. IL-10 mRNA expression in lung tissue of PQ group was elevated at the six hour, reached the peak at the first day, at the third day [（0.551±0.016） and （0.524±0.010） respectively] and the seventh day also higher than that in normal control group. At the first and the seventh day in the PQ group it was significantly higher than that in normal control group（P〈0.01）. Meanwhile, HMGB-1 mRNA expression in lung tissue of PQ group was also elevated at the six hour, reached the peak at the first day, at the third [（0.695±0.060）,（0.871±0.154） and（0.819±0.188） respectively] and the seventh day also higher than that in normal control group. At six hour, the first and the third day in the PQ group it was significantly higher than that in normal control group （P〈0.01）. The histological change

关 键 词：百草枯 肿瘤坏死因子 白细胞介素-10 

分 类 号：R686[医药卫生—骨科学]